7600 fully automated biochemical analyzer

A Hitachi 7600 fully automated biochemical analyzer was used to detect serum ALT, total bilirubin, and albumin.

Cystatin C (Cys C) was assayed using enzyme-linked immunosorbent assay (ELISA) kits purchased from Crystal Chem Inc. (Elk Grove Village, IL, USA); mouse kidney injury molecule 1 (KIM-1) was assayed using ELISA kits purchased from Shanghai Walan Biotechnology Co., Ltd. (Shanghai, China). Blood urea nitrogen (BUN) and serum creatinine (Scr) were measured on a 7600 fully automated biochemical analyzer (Hitachi, Beijing, China).

Written informed consent was obtained from all participants enrolled in the present study. The study protocol was approved by the Ethics Committee of The First Affiliated Hospital at the Guangxi Medical University (Nanning, China). A total of 7 ml of blood was collected from the proband and 95 healthy volunteers (2 ml of blood was used for EDTA anticoagulation and 5 ml was used for separation of coagulation factors). The proband (3 year old female) was recruited following routine surgery at the Department of Clinical Laboratory, The First Affiliated Hospital of Guangxi Medical University, and all participants (57 males and 38 females; mean age of 23.15±11.59 years; age range of 7–51 years) in the present study were recruited between January 2016 and December 2016 at The First Affiliated Hospital of Guangxi Medical University.
Blood tests, including RBC count, hemoglobin (HGB) levels, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean sphered blood cell volume (MSCV), mean reticulocyte volume, reticulocyte (RET) levels and mean platelet volume (MPV), were performed using the LH 780 automated hematology analyzer (Beckman Coulter, Inc., Brea, CA, USA). For assessment of liver function, levels of total bilirubin (TBiL) and direct bilirubin (DBiL) were determined using the 7600 Fully Automated Biochemical Analyzer (Hitachi, Ltd., Tokyo, Japan).