The HCC cell line Mahlavu was kindly provided by dr. Rengul Cetin-Atalay (Bilkent University, Ankara, Turkey) [65 –67 (link)] while Hep3B (ATCC no: HB-8064), HepG2 (ATCC no: HB-8065) SNU449 (ATCC no: CRL-2234) and SNU475 (ATCC no: CRL-2236) were obtained from ATCC. Mahlavu, Hep3B and HepG2 were maintained in DMEM medium supplemented with 10% FBS, 2 mM L-Glutamine, 0.1 mM NEAA, 100 U/ml penicillin and 100 μg/ml streptomycin. SNU449 and SNU475 cell lines were maintained in RPMI-1640 medium supplemented with 10% FBS, 2 mM L-Glutamine, 100 U/ml penicillin and 100 μg/ml streptomycin. For normoxic condition, all cells were cultured in a 37°C humidified incubator and an atmosphere of 5% CO2 in air. For hypoxic condition, cells were cultured in a CO2 incubator (Forma™ Series II Water Jacket CO2, Thermo Scientific, USA) maintained at 94% N2, 5% CO2 and 1% O2 for indicated times of treatments. Western blot analysis was performed by standard methods as described elsewhere [68 (link)–70 (link)].
Forma series 2 water jacket co2
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Forma™ Series II Water Jacket CO2 Incubator is a laboratory equipment designed for cell culture applications. It maintains a controlled environment with temperature, CO2 concentration, and humidity regulation. The incubator utilizes a water jacket system for uniform temperature distribution.
Automatically generated - may contain errors
Lab products found in correlation
Snu 449, by American Type Culture Collection (1 mentions)
Hep3b, by American Type Culture Collection (1 mentions)
Hepg2, by American Type Culture Collection (1 mentions)
Snu475, by American Type Culture Collection (1 mentions)
Fetal bovine serum (fbs), by R&D Systems (1 mentions)
L ascorbic acid, by Thermo Fisher Scientific (1 mentions)
Basic fibroblast growth factor (bfgf), by R&D Systems (1 mentions)
Ascorbic acid, by Merck Group (1 mentions)
2 protocols using forma series 2 water jacket co2
1
Hepatocellular Carcinoma Cell Lines
2
Optimizing Chondrocyte Culture Conditions
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Human or rabbit chondrocytes were seeded in 25 cm2 cultured flasks in a humidified Thermo Scientific Forma Series II Water Jacket CO2 incubator in chondrocyte culture medium: Ham's F12 culture medium with l -glutamine, 25 μg mL−1 of l -ascorbic acid, 100 units per mL of penicillin, 100 μg mL−1 of streptomycin, 0.25 μg mL−1 of Fungizone® Antimycotic (AM) and 10% FBS. The culture medium was changed twice a week. To optimize the culture, an MTS assay was performed with different chondrocyte medium compositions: Ham's F12, 1% AM (Invitrogen, Life Technologies, Carlsbad, CA, USA), 25 μg mL−1 ascorbic acid (Sigma-Aldrich, USA), different concentrations of fetal bovine serum (FBS, Lonza, Walkersville, MD, USA) and the presence or absence of basic fibroblast growth factor (b-FGF at 10 ng mL−1, R&D Systems Inc, Minneapolis, Minn, USA) to find the best option for chondrocytes culture (10% FBS + b-FGF). MSC were cultured with DMEM media supplemented with 100 units per mL of penicillin, 100 μg mL−1 of streptomycin, 0.25 μg mL−1 of AM and 10% FBS.
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