Cary 50 probe uv visible spectrophotometer

The optical rotation was measured on a JASCO P-1010 (JASCO, Easton, MD, USA). UV data were recorded on a CARY 50 Probe UV-visible spectrophotometer (Agilent Technologies, Santa Clara, CA, USA). The IR spectra were recorded using a PerkinElmer FTS FT-IR spectrophotometer (PerkinElmer, Shelton, CT, USA). Electronic circular dichroism (ECD) spectra were recorded on a JASCO J-815 CD spectropolarimeter (JASCO, Easton, MD, USA). The NMR spectra were recorded on a Bruker AV400 (400 MHz for ¹H-NMR, 100 MHz for ¹³C-NMR; Bruker, Billerica, MA, USA) using tetramethylsilane (TMS) as an internal standard. Silica gel G60 (60–200 mm; SiliCycle Inc., Quebec, QC, Canada), and Sephadex LH-20 (18–111 µm, GE Healthcare, Chicago, IL, USA) were used for column chromatography (CC). HRESIMS spectra were obtained with a Bruker micrOTOF-Q II (Bruker, Billerica, MA, USA). TLC normal and TLC reverse phase analysis were performed on Silicycle’s aluminum sheet coated with silica gel F-254 (SiliCycle Inc., Quebec, QC, Canada) and on Merck’s aluminum sheets coated with silica gel 60 RP-18 F₂₄₅s (Merck, Rahway, NJ, USA), respectively.

The cell biomass of RTU culture was collected by centrifugation at 10,000 × g for 15 min (Hitachi Himac CR22611 High-Speed Refrigerated Centrifuge, Hitachi Koki Co Ltd., Tokyo, Japan) and washed with sterile 0.85% (w/v) NaCl solution (Merck, Darmstadt, Germany). The cell population of RTU suspension was adjusted to approximately 10⁹ CFU/ml using sterile 0.85% (w/v) NaCl solution [Absorbance (Abs) of 1.00 at 600 nm wavelength (Cary 50 Probe UV-visible spectrophotometer, Agilent Technologies, Santa Clara, CA, USA) (16 (link))].