High-resolution, cross-sectional images of injected hind limbs were obtained using a VivaCT 40 (Scanco, Nokomis, FL, http://www.scanco.com/ ) at a source voltage of 55 kV, a source current of 142 µA, and an isotropic voxel size of 38.0 µm. A three-dimensional (3D) image was reconstructed using Scanco microCT V6.1 software. The skeletal bone of the hind limbs and the sites of ectopic ossification were imaged separately, using two different thresholds to optimize visualization and quantification of HEO formation. The optimal threshold for the skeletal bone was a lower threshold of 212 Hounsfield and an upper threshold of 1,000 Hounsfield units. The optimal threshold for detecting ectopic ossification was a lower threshold of 150 Hounsfield and an upper threshold of 1,000 Hounsfield units. Detected ectopic mineralization was quantified using Scanco microCT V6.1 software.
Microct v6
Manufactured by Scanco
MicroCT v6.1 is a software for analysis and visualization of 3D micro-computed tomography (micro-CT) data. It provides tools for reconstructing, segmenting, and analyzing high-resolution 3D images obtained from micro-CT scans.
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Lab products found in correlation
3 protocols using microct v6
1
Quantifying Ectopic Ossification in Hind Limbs
2
Microcomputed Tomography Analysis of Maxillary Bone Density
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The harvested maxillae were fixed with 4% paraformaldehyde at 4°C overnight and stored in a 70% ethanol solution. The fixed maxillae were scanned by a microcomputed tomography scanner (microCT35; Scanco Medical AG, Bassersdorf, Switzerland) with a 14 µm voxel size. Two-dimensional slices from each maxilla were combined using microCT v6.1 software (Scanco Medical) to form a three-dimensional reconstruction. Reconstructions were further analysed as previously described18 (link). The region of interest (ROI) was defined to cover the whole tooth extraction socket. The bone mean density (BMD) of each extraction site was measured semi-automatically in accordance with the guidelines for the assessment of bone microstructures using microCT37 (link).
3
Longitudinal In Vivo Microcomputed Tomography
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Longitudinal in vivo microcomputed tomography (μCT) imaging was performed at indicated timepoints using a VivaCT40 (Scanco, Nokomis, FL, United States) at a source voltage of 55 kV, a source current of 145 μA, and an isotropic voxel size of 38.0 μm. Mice were imaged under isoflurane anesthesia. Only the lower limb was imaged to reduce radiation exposure from multiple scans. Three-dimensional images were reconstructed using Scanco microCT V6.1 software. For quantification, the heterotopic bone was reconstructed separately from skeletal bone, with a lower threshold of 220 Hounsfield and an upper threshold of 1,000 Hounsfield units (Culbert et al., 2014 (link)).
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