Mouse anti flag antibody a8592

Manufactured by Merck Group

The Mouse anti-FLAG antibody (A8592) is a monoclonal antibody that recognizes the FLAG tag, a commonly used molecular tag. The antibody is designed for use in various laboratory techniques, such as Western blotting, immunoprecipitation, and immunoaffinity chromatography, to detect and purify proteins tagged with the FLAG sequence.

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Lab products found in correlation

Anti pcna sc 56, by Santa Cruz Biotechnology (2 mentions) Anti mouse alexa fluor 488, by Thermo Fisher Scientific (2 mentions) Rabbit anti fus, by Fortis Life Sciences (2 mentions) Anti lig1 a301 136a, by Fortis Life Sciences (2 mentions) Mouse anti lig3 antibody, by Abcam (2 mentions) Texas red anti rabbit antibody, by Thermo Fisher Scientific (2 mentions)

2 protocols using mouse anti flag antibody a8592

Mitochondrial Targeting of Lig1 Protein

Cited 1 time

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Rabbit anti-FUS (Cat# A300-302A) and anti-Lig1 (A301-136A) antibodies were procured from Bethyl Laboratories, Inc. Mouse anti-FLAG antibody (A8592) was obtained from Sigma-Aldrich, and mouse anti-Lig3 antibody (Cat# ab587) was purchased from Abcam. Mouse anti-Tom20 (SC-17764), anti-HSP60 (SC-13115) and anti-PCNA (SC-56) antibodies were procured from Santa Cruz Biotechnology. Fluorescent secondary antibodies, Alexa Fluor 488 anti-mouse (Cat# A28175), and Texas Red anti-rabbit antibody (Cat# T-2767) were obtained from Life Technologies. The antibodies were diluted at 1:1000 for western blotting, 1:500 for immunofluorescence and 1:100 for PLA.
The human Lig1 coding sequence was re-cloned into pCDNA3.1 plasmid as an N-terminal FLAG and COX8 gene mitochondrial targeting sequence containing construct (FLAG-MTS-Lig1) ^{53 (link)}.

Kodavati M., Wang H., Guo W., Mitra J., Hegde P.M., Provasek V., Maloji Rao V.H., Vedula I., Zhang A., Mitra S., Tomkinson A.E., Hamilton D.J., Bosch L.V, & Hegde M.L. (2023). FUS Unveiled in Mitochondrial DNA Repair and Targeted Ligase-1 Expression Rescues Repair-Defects in FUS-Linked Neurodegeneration. Research Square.

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Mitochondrial Ligase 1 Localization

Cited 1 time

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Rabbit anti-FUS (Cat# A300–302 A) and anti-Lig1 (A301-136A) antibodies were procured from Bethyl Laboratories, Inc. Mouse anti-FLAG antibody (A8592) was obtained from Sigma-Aldrich, and mouse anti-Lig3 antibody (Cat# ab587) was purchased from Abcam. Mouse anti-Tom20 (SC-17764), anti-HSP60 (SC-13115) and anti-PCNA (SC-56) antibodies were procured from Santa Cruz Biotechnology. Rabbit anti-CYTB (Cat# 55090-1-AP), COX1 (Cat# 13393-1-AP) and ND4 (Cat# 26736-1-AP) procured from Protein tech. Fluorescent secondary antibodies, Alexa Fluor 488 anti-mouse (Cat# A28175), and Texas Red anti-rabbit antibody (Cat# T-2767) were obtained from Life Technologies. The antibodies were diluted at 1:1000 for western blotting, 1:500 for immunofluorescence, and 1:100 for PLA.
The human Lig1 coding sequence was re-cloned into pCDNA3.1 plasmid as an N-terminal FLAG and COX8 gene mitochondrial targeting sequence containing construct (FLAG-MTS-Lig1)^{56 (link)}.

Kodavati M., Wang H., Guo W., Mitra J., Hegde P.M., Provasek V., Rao V.H., Vedula I., Zhang A., Mitra S., Tomkinson A.E., Hamilton D.J., Van Den Bosch L, & Hegde M.L. (2024). FUS unveiled in mitochondrial DNA repair and targeted ligase-1 expression rescues repair-defects in FUS-linked motor neuron disease. Nature Communications, 15, 2156.

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