Luna omega ps 1.6 m

Polyphenols were extracted from lyophilized micro-greens with methanol/water (60:40, v/v) and analyzed according to the method detailed in Kyriacou et al. [17 (link)]. Analysis was performed using a UHPLC system (Dionex UltiMate 3000, Thermo Fisher Scientific, Waltham, MA, USA) coupled to a Q-Exactive Orbitrap mass spectrometer (UHPLC, Thermo Fischer Sci-entific, Waltham, MA, USA). Polyphenols were separated using a Luna Omega PS 1.6 µm (50 mm × 2.1 mm, Phenomenex) column. Identification and quantification of compounds was accomplished using a Q-Exactive Orbitrap mass spectrometer (UHPLC, Thermo Fischer Scientific, Waltham, MA, USA) operated in fast negative/positive ion switching mode (Thermo Scientific, Bremen, Germany). Data analysis and processing were performed using software Xcalibur v. 3.0.63 (Xcalibur, Thermo Fisher Scientific, Waltham, MA, USA). Phenolic compounds were identified and quantified against calibration curves of available standards and expressed in µg g⁻¹ DW. For standards that were not available, identification MS/MS experiments were employed.