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Ix71 microscopy system

Manufactured by Olympus
Sourced in Japan

The IX71 microscopy system is a versatile and high-performance microscope from Olympus. It is designed for a wide range of research and analysis applications. The IX71 offers advanced optical and illumination capabilities to support various imaging techniques.

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2 protocols using ix71 microscopy system

1

Wound Healing Assay in Endometrial Cancer Cells

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Uniform wounds were introduced into cell cultures using a culture insert (#80201; ibidi, Munich, Germany). The inserts were placed in individual wells of a six-well plate. Ishikawa, HEC-1B and AN3CA cells transfected with vector for 96 h or AN3CA cells treated with sitagliptin or left untreated were seeded in each reservoir of the insert (1×104cells in a finalvolume of 100 μl). Inserts were removed after cells adhered. The gaps (wounds) were washed with serum-free medium, and 2ml fresh medium were added to each well. AN3CA cells were treated with 1 mMsitagliptin, with culture medium aloneserving as a control. Cell migration into the wound area was recorded using an IX71 microscopy system coupled to a DP73 digital camera (both from Olympus, Tokyo, Japan) at 0 and 48 h. Wound closure at 24–48 h was compared with time 0.
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2

ER1 and PR1 Expression in EC Cells

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We confirmed ER1 and PR1 expression in all EC cell lines via qRT-PCR as compared with analogous data provided by ATCC. EC cells morphologies before and after DPPIV up- or downregulation were also recorded using an IX71 microscopy system coupled to a DP73 digital camera (both from Olympus, Tokyo, Japan).
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