Mouse monoclonal antibody against actin mab1501

Manufactured by Merck Group

Mouse monoclonal antibody against actin (#MAB1501) is a laboratory reagent that specifically binds to the actin protein. It is a purified immunoglobulin G (IgG) class antibody produced in mice.

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Mouse monoclonal antibody against actin Actin (MAB1501, Antibody against actin Monoclonal mouse Actin Monoclonal actin antibody Monoclonal mouse antibody MAB1501 Monoclonal mouse

2 protocols using mouse monoclonal antibody against actin mab1501

Lentiviral-Mediated Myr-AKT1 Expression

Cited 1 time

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Lentiviral expression for Myr-AKT1 was previously described (15 (link)). Antibodies specific to p-IGFIRβ/InsRβ (#3024), IGFIRβ (#3018), pY1068-EGFR (#3777), EGFR (#4267), pY705-STAT3 (#9145), STAT3 (#9139), pS473-AKT (#4060), AKT (#2920), p-ERK (#4370), ERK (#4696) and cleaved-caspase 3 (#9661) were purchased from Cell Signaling Technologies. Monoclonal antibody against InsRβ (sc-57342) was purchased from Santa Cruz Biotechnology. Mouse monoclonal antibody against actin (#MAB1501) was purchased from Millipore.
Gefitinib (G-4408) and OSI-906 (L-5814) was purchased from LC Laboratories. BMS-754807 (CT-BMS75) was purchased from Chemitek. Dacomitinib (S2727) was purchased from Selleckchem. Recombinant Human IGF1 (AF-100-11), Heregulin-β1 (#100-03), HGF (#100-39), PDGF-AB (100-00AB), were from Peprotech. Insulin (#12585-014) was from Life technology.

Ma Y., Tang N., Thompson R., Mobley B.C., Clark S.W., Sarkaria J.N, & Wang J. (2015). InsR/IGF1R pathway mediates resistance to EGFR inhibitors in glioblastoma. Clinical cancer research : an official journal of the American Association for Cancer Research, 22(7), 1767-1776.

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Evaluating Notch1 and EGFR Signaling

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NXD30001 was obtained from NexGenix Pharmaceuticals, 17AAG was generously provided by Kosan Bioscience, Inc. The antibodies used in this study including Phospho(p)-EGFR (1H12, #2236), EGFR (D6B6, #2085), pS473-Akt (D9E, #4060), Akt (40D4, #2920), Cleaved-Notch1 (D3B8, #4147), Notch1 (D1E11, #3608), c-Myc (D84C12, #5605), p-MEK (Thr286, #9127), p-ERK1/2 (D13.14.4E, #4370), ERK1/2 (L34F12, #4696), p-ATR (Ser428, #2853), p-CHK2 (Thr68, #2661), p-p53 (Ser15, #9286), p-H2AX (D7T2V, #80312), PERK (D11A8, #5683), IRE1α (14C10, #3294), BiP (C50B12, #3177), and CHOP (L63F7, #2895) were purchased from Cell signaling Technology. Mouse monoclonal antibody against actin (#MAB1501) was purchased from Millipore. Secondary antibodies were obtained from Santa Cruz Biotechnology, Inc.

Chen H., Gong Y., Ma Y., Thompson R.C., Wang J., Cheng Z, & Xue L. (2020). A Brain-Penetrating Hsp90 Inhibitor NXD30001 Inhibits Glioblastoma as a Monotherapy or in Combination With Radiation. Frontiers in Pharmacology, 11, 974.

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