720 es icp oes spectrophotometer

Dry matter, crude ash, crude protein, crude fat, and crude fibre were determined in the diets [16 ]. Amino acids were analysed with a Sykam Amino Acid Analyzer (Laserchrom HPLC Laboratories Ltd. Inc., Rochester, UK) according to the AOAC procedure [16 ]. The content of calcium was determined by atomic absorption spectrometry with a Varian model 720-ES ICP-OES spectrophotometer (Varian, Palo Alto, USA), Total P content in the feed was determined colorimetrically with a Helios Alpha UV-Vis spectrophotometer (Spectronic Unicam, Leeds, UK). Also determined in the diets were the content of phytate phosphorus, lactic acid, glucosinolates and tannins [14 (link)].
Phytate was determined colorimetrically according Vaintraub and Lapteva [17 (link)]. The method of determining lactic acid was based on colorimetric determination of its content by the LA-Fe (III) complex method [18 (link)]. Glucosinolate content in sampls was estimated according to the standard PN-ISO 10633–1 [19 ] using high performance liquid chromatography. The Folin-Denis spectrophotometric method was used to determine the tannin content according to Canbaş et al. [20 (link)] in modification.

Tests from Cormay (Lublin, Poland) were used to analyse the plasma for selected biochemical parameters, i.e., total protein (TP; Cor-TOTAL PROTEIN 60, catalogue No. 2-236, Poland), uric acid (UA; Liquick Cor-URIC ACID 120, catalogue No. 2-208, Poland), urea (UREA; Liquick Cor-UREA 60, catalogue No. 2-206, Poland), creatinine (CREAT; Liquick Cor- CREATININE 60, catalogue No. 2-233, Poland), phosphorus (P; Liquick Cor-PHOSPHORUS 120, catalogue No. 2-338, Poland); total cholesterol (CHOL; Liquick Cor-CHOL 60, catalogue No. 2-204, Poland), triacylglycerols (TG; Liquick Cor-TG 30, catalogue No. 2-262, Poland), and HDL cholesterol (HDL-C; Liquick Cor-HDL, catalogue No. 2-053, Poland). LDL cholesterol (LDL-C) was calculated using the formula given by Friedewald [24 (link)]:
Tests from Cormay (Lublin, Poland) were used to determine the activity of alkaline phosphatase (ALP; Liquick Cor-ALP 60, catalogue No. 1-212, Poland), alanine aminotransferase (ALT; Liquick Cor-ALAT 60, catalogue No. 1-216, Poland), and aspartate aminotransferase (AST; Liquick Cor-ASAT 60, catalogue No. 1-214, Poland).
Ca, Mg, Zn, Cu, and Fe content was determined by atomic absorption spectrometry (AAS) with a Varian model 720-ES ICP-OES spectrophotometer (Varian, Palo Alto, Santa Clara, CA, USA).