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2 protocols using mdl12

1

HBV Entry Inhibitors and Molecular Probes

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Paraformaldehyde (PFA), Triton X-100, Tunicamycin, Cytochalasin D, Latrunculin A, Colchicine, Demecolchine, Taxol, cAMP, Wortmannin, MDL12, Caffeine and Roflumilast were obtained from Sigma-Aldrich. DMSO was from VWR International. For detection of the NTCP protein, an anti-FLAG antibody (DYKDDDDK Epitope Tag antibody; ThermoFisher Scientific) was used, while for the detection of HBV core particles, an anti-HBcAg was ordered from Dako, Agilent Technologies Antibodies against β-actin and γ-Tubulin were from Sigma-Aldrich, while PCNA was obtained from Abcam. Small interference RNAs (siRNAs) were ordered from ThermoFisher Scientific. cAMP direct immunoassay kit was purchased from Abcam. Human phospho-kinase arrays were performed according to the manufacturer's instructions (R&D Systems).
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2

Inhibitor Compound Acquisition and Use

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GF109203X (GFX), H89, MDL-12,330A, EGTA, repaglinide, STO-609, KN93, and nifedipine were purchased from Sigma-Aldrich. BBT was obtained from a commercial compound library (Specs, Zoetermeer, the Netherlands).
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