DPPs (Scheme 1 , Additional file 1 : Table S1, S2 and Fig. S1–S3) were synthesized by ChinaPeptides Co., Ltd. (Suzhou, China). 2′-O-methyl (2′-OMe) modified antisense oligonucleotides (ASOs), 5′-fluorescein amidites (FAM)-labeled ASOs and primers were synthesized by Sangon Biotech (Shanghai, China) (Additional file 1 : Fig. S3). 5′-Cyanine 5 (Cy5)-labeled ASOs were synthesized by Tsingke Biological Technology Co., Ltd (Xi’an, China). 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-methoxy(polyethylene glycol)-2000 (DSPE-mPEG2000) was purchased from Xi'an ruixi Biological Technology Co., Ltd. Trizol (Additional file 1 : Fig. S3), FM4-64 dye, Lipofectamine 2000™ and Opti-MEM medium were purchased from Life Technologies (Invitrogen, CA, USA). Lysogeny broth and Mueller–Hinton (M–H) broth were purchased from Beijing Land Bridge Technology Co., Ltd (Beijing, China). Reversed enzyme and SYBER Green enzyme were purchased from Takara Bio Inc. (Kyoto, Japan).
Reversed enzyme
Manufactured by Takara Bio
Sourced in Japan
The Reversed Enzyme is a laboratory equipment designed to perform the reverse transcription of RNA into complementary DNA (cDNA). It is a crucial tool used in various molecular biology applications, such as gene expression analysis, reverse transcription-polymerase chain reaction (RT-PCR), and cDNA library construction.
Automatically generated - may contain errors
Lab products found in correlation
1 2 distearoyl sn glycero 3 phosphoethanolamine n methoxy polyethylene glycol 2000 dspe mpeg2000, by Xi’an Ruixi (6 mentions)
Opti mem medium, by Thermo Fisher Scientific (2 mentions)
Fm4 64 dye, by Thermo Fisher Scientific (2 mentions)
5 cyanine 5 cy5 labeled asos, by Tsingke (2 mentions)
Syber green enzyme, by Takara Bio (2 mentions)
Trizol, by Thermo Fisher Scientific (2 mentions)
Mueller hinton broth, by Beijing Land Bridge Technology (2 mentions)
Lysogeny broth, by Beijing Land Bridge Technology (2 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 2000tm, by Thermo Fisher Scientific (1 mentions)
2 protocols using reversed enzyme
1
Synthesis and Characterization of Peptide-Based Therapeutics
2
Synthesis and Labeling of Peptides
Check if the same lab product or an alternative is used in the 5 most similar protocols
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DPPs (Scheme 1, Additional le 1: Table S1-S2 and Figure S1-S3) were synthesized by ChinaPeptides Co., Ltd. (Suzhou, China). 2′-O-methyl (2′-OMe) modi ed antisense oligonucleotides (ASOs), 5′-uorescein amidites (FAM)-labeled ASOs and primers were synthesized by Sangon Biotech (Shanghai, China) (Additional le 1: Figure S3). 5′-Cyanine 5 (Cy5)-labeled ASOs were synthesized by Tsingke Biological Technology Co., Ltd (Xi'an, China). 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-Nmethoxy(polyethylene glycol)-2000 (DSPE-mPEG2000) was purchased from Xi'an ruixi Biological Technology Co., Ltd. Trizol (Additional le 1: Figure S3), FM4-64 dye, Lipofectamine 2000 TM and Opti-MEM medium were purchased from Life Technologies (Invitrogen, CA, USA). Lysogeny broth and Mueller-Hinton (M-H) broth were purchased from Beijing Land Bridge Technology Co., Ltd (Beijing, China). Reversed enzyme and SYBER Green enzyme were purchased from Takara Bio Inc. (Kyoto, Japan).
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