Spinning disk confocal microscopy was performed using a Yokogawa CV1000 equipped with an Olympus 100X NA1.40 UPLSAPO WD 0.13 (mm) oil immersion objective, 50 μm Nipkow microlens disk, 488 nanometer excitation laser, and a high-resolution format Hamamatsu Photonics ImagEM X2 EM-CCD (C9100-14) camera with 1024 × 1024 pixels. The emission filter (Olympus) used was 525/50 nm (GFP). Cells were brought into focus using a 790 nm laser diode auto-focusing system and single images through the center of the cells were acquired at full camera resolution. All images for comparison were acquired with identical settings using the CV1000 software and were adjusted identically using Fiji (ImageJ) for presentation purposes. For all strains and conditions, at least ten random fields of view were imaged, representative cells are shown.
Imagem x2 em ccd c9100 14 camera
Manufactured by Hamamatsu Photonics
The ImagEM X2 EM-CCD (C9100-14) camera is a high-performance electron-multiplying charge-coupled device (EM-CCD) camera designed for low-light imaging applications. It features a 512x512 pixel sensor with a 16-bit analog-to-digital converter and a pixel size of 16 x 16 μm. The camera can achieve a maximum readout speed of 20 MHz and a maximum frame rate of 30 frames per second. It is equipped with an on-chip electron multiplication gain function, which enhances the signal-to-noise ratio for improved detection of weak signals.
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2 protocols using imagem x2 em ccd c9100 14 camera
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Spinning Disk Confocal Microscopy Imaging
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Spinning Disk Confocal Microscopy Imaging
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Spinning disk confocal microscopy was performed using a Yokogawa CV1000 equipped with an Olympus 100X NA1.40 UPLSAPO WD 0.13 (mm) oil immersion objective, 50 μm Nipkow microlens disk, 488 nanometer excitation laser, and a high-resolution format Hamamatsu Photonics ImagEM X2 EM-CCD (C9100-14) camera with 1024 × 1024 pixels. The emission filter (Olympus) used was 525/50 nm (GFP). Cells were brought into focus using a 790 nm laser diode auto-focusing system and single images through the center of the cells were acquired at full camera resolution. All images for comparison were acquired with identical settings using the CV1000 software and were adjusted identically using Fiji (ImageJ) for presentation purposes. For all strains and conditions, at least ten random fields of view were imaged, representative cells are shown.
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