Anti il 12 rβ2 305719

Antibodies used for immunoblotting, cell-surface, and intracellular stains were purchased from commercial sources. The anti-LAT Y191 (C305) was from Millipore. The anti-LCK pY505 (4/Lck (pY505), anti-SLP-76 pY128 (J141-668.36.58), and anti-IL-12Rβ1 (114) were from BD Biosciences. The anti-PLC-γ1 pY783 (polyclonal), anti-p38 pT180/Y182 (3D7), anti-p38 (polyclonal), anti-AKT pT308 (244F9), anti-ZAP-70 pY319 (polyclonal), anti-SRC pY416 (polyclonal), anti-STAT4 (2A2), anti-JNK T183/Y185 (polyclonal), anti-MKK3/MKK6 pS189/S207 (D8E9), and anti-STAT4 pY693 (D2E4) antibodies were purchased from Cell Signaling Technologies. The anti-ERK1/2 pTpY185/187 (polyclonal) was from Invitrogen. The anti-SOS1 (polyclonal) and anti-CD3-ζ (6B10.2) from Santa Cruz Biotechnology. The anti-GAPDH was from Meridian Life Science. The DyLight 800 and DyLight 680 labeled secondary antibodies were obtained from Thermo Scientific. The FITC anti-IFN-γ (4S.B3), APC anti-TNF-α (Mab11), anti-CD3 (OKT3), anti-CD28 (CD28.2), anti-CD2 (RPA-2.10), anti-CD49d (9F10), anti-CD11a (HI111) from BioLegend. Anti-IL-12 Rβ2 (305719) was purchased from R&D Systems. The anti-mouse IgG was from Southern Biotech.

Anti-CD3 (145–2C11), anti-CD4 (RM4–5), anti-CD8 (53–6.7), anti-CD25 (7D4), anti-CD44 (IM7), anti-CD45 (30-F11), anti-Pax5 (1H9), anti-CD45.1 (A20), anti-CD11b (M1/70), anti-CD11c (HL3), anti-CD117 (2B8), anti-IFNγR1 (XMG1.2), anti-pSTAT6^Y641 (J71–773.58.11), anti-pSTAT3^S727 (49/p-Stat3), anti-pSTAT3^Y705 (4/P-STAT3), anti-Zbtb46 (U4–1374), anti-SIRPα (P84), and anti-CD90.1 (OX-7), were purchased from BD biosciences (San Jose, CA). Anti-pSTAT1^S727 (D3B7), and anti-pSTAT1^Y701 (D4A7) antibodies were purchased from Cell Signaling Technologies (Danvers, MA). Anti-CD45.2 (104), anti-IRF-8 (V3GYWCH), and anti-IRF-4 (3E4) were purchased from eBiosciences (San Diego, CA). Anti-PU.1 (7C2) was purchased from Biolegend (San Diego, CA). Anti-IL-12Rβ2 (305719) was purchased from R&D Systems (Minneapolis, MN), Anti-IL-13Rα1 antibody (1G3-A7) was produced in our laboratory (16 (link)).