To produce sufficient quantities of human AGT, the protein was produced as a fusion with a SUMO tag to increase solubility, i.e. the 6×His-SUMO-AGT construct. Limited tryptic digestion was performed at a 50 : 1 substrate:trypsin (Promega) weight ratio at 37°C. Digestion was stopped by addition of 0.1% formic acid and samples were processed on the same day. Peptide design was guided by solubility analysis and sequences used for epitope confirmation were P1: ANAGKPKDPTFI (amino acid no. 24–35), P2: PAPIQAKTSPVD (36–47), P3: PVDEKALQDQ (45–54), P4: LVAAKLDTED (55–66), P5: EDKLRAAMVG (65–74), P6: NFLGFRIYGMHSE (78–90), P7: HTADRLQAIL (120–129) and P8: AILGVPWKDK (127–136). Solid-phase synthesised peptides were ordered from Mimotopes Pty Ltd (Australia) with purity >90%. The peptides were dissolved in double-distilled water to a concentration of 1 mM and 2 ml were used for dot blot analysis.
Solid phase synthesised peptides
Manufactured by Mimotopes
Sourced in Australia
Solid-phase synthesised peptides are chemically synthesised peptide sequences produced using a solid-phase synthesis technique. This method allows for the construction of customised peptide chains by sequentially adding amino acids to a solid support.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using solid phase synthesised peptides
1
Recombinant Human AGT Protein Purification
2
Recombinant Human AGT Protein Purification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To produce sufficient quantities of human AGT, the protein was produced as a fusion with a SUMO tag to increase solubility, i.e. the 6×His-SUMO-AGT construct. Limited tryptic digestion was performed at a 50 : 1 substrate:trypsin (Promega) weight ratio at 37°C. Digestion was stopped by addition of 0.1% formic acid and samples were processed on the same day. Peptide design was guided by solubility analysis and sequences used for epitope confirmation were P1: ANAGKPKDPTFI (amino acid no. 24–35), P2: PAPIQAKTSPVD (36–47), P3: PVDEKALQDQ (45–54), P4: LVAAKLDTED (55–66), P5: EDKLRAAMVG (65–74), P6: NFLGFRIYGMHSE (78–90), P7: HTADRLQAIL (120–129) and P8: AILGVPWKDK (127–136). Solid-phase synthesised peptides were ordered from Mimotopes Pty Ltd (Australia) with purity >90%. The peptides were dissolved in double-distilled water to a concentration of 1 mM and 2 ml were used for dot blot analysis.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!