Arium pro uv ultrapure water system

LC–MS grade water, methanol (MeOH), and 2-propanol (IPA) were purchased from Honeywell (Chromasolv™ HPLC solvents series, Bucharest, Romania). Formic acid (FA; 98–100%, Suprapur®) was purchased from Merck Millipore (Darmstadt, Germany). Norleucine (98% purity), naringenin (98% purity), nicotinamide (≤ 99.5% purity), and ααα-trifluoro-m-toluic acid (99% purity) were purchased from Sigma-Aldrich (Vienna, Austria). Ultrapure water for pre-cleaning of apparatuses was prepared with arium® pro UV ultrapure water system (Sartorius, Goettingen, Germany) equipped with arium® analytical kit (Sartorius) and Sartopore® 2 capsule 0.2 µm (Sartorius).

The solution used for the EPD process consisted of two solvents, namely, methanol, and water. The methanol (CH₃OH) was supplied by Merck kGaA (Darmstadt, Germany), whereas water was obtained using an Arium Pro UV Ultrapure water system (Sartorius, Göttingen, Germany). The MWCNTs (Product No. 0552CA) were from SkySpring Nanomaterials, Inc. (Houston, TX, USA). These MWCNTs were 95% purity, <8 nm in size, and in black powder form.
The carbon fibers used in the experiment were black plain weave CF supplied from PiCarbon (Kuala Lumpur, Malaysia). In addition, Epocast PT100 (clear type epoxy resin) and Epoharden PT100S (amine-based epoxy hardener) were obtained from Portal Trading (P.T.) (Pulau Pinang, Malaysia) and were used as matrix and curing agents, respectively.

Purified water was obtained from an Arium Pro UV ultrapure water system (Sartorius Stedim Biotech, Goettingen, Germany). LC-MS grade water containing 0.1% (V/V) formic acid (FA) and LC-MS grade acetonitrile containing 0.1% (V/V) FA were obtained from Fisher Scientific (Dublin, Ireland). All reagents and materials required for digestion such as Thermo Scientific™ SMART Digest™ trypsin kit, Thermo Scientific™ KingFisher Deepwell 96-well plates, and 12-tip combs were obtained from Thermo Fisher Scientific (Sunnyvale, CA, USA). DL-dithiothreitol (DTT) (≥98%), iodoacetamide (IAA), Trizma® base, phosphate-buffered saline (PBS), and glycine were purchased from Sigma-Aldrich (Wicklow, Ireland). Hi3 Phos B standard for label-free Hi3 quantitation of detected HCPs was obtained from Waters (Wexford, Ireland). A commercially available IgG1, rituximab, was provided by the Pharmacy Unit of the University Hospital of San Cecilio in Granada, Spain.