C4–2B MDVR (C4–2B Enzalutamide resistant) and CWR22Rv1 cells were maintained in RPMI1640, whereas HEK293 and IMR90 fibroblast cells were cultured in DMEM supplemented with 10% fetal bovine serum (FBS), 100 units/ml penicillin, and 0.1 mg/ml streptomycin. RWPE-1 cells were grown in Karotinocyte SFM medium (Gibco). All cell line experiments were performed within six months of receipt from the ATCC or resuscitation after cryopreservation. C4–2B cells were kindly provided and authenticated by Dr. Leland Chung Lab at Cedars-Sinai Medical Center (Los Angeles, CA, USA). C4–2B MDVR cells were maintained in medium containing 20 μM Enzalutamide. Parental C4–2B cells were passaged alongside the resistant cells as an appropriate control [25 (link),26 ]. All cell lines were routinely tested as mycoplasma-free by PCR and authenticated using the short tandem repeat (STR) method. All cells were maintained at 37 °C in a humidified incubator with 5% carbon dioxide. Enzalutamide, apalutamide, darolutamide, and abiraterone acetate were purchased from Selleck Chemical. JG98 and JG231 were synthesized as described and their identities confirmed by 1H NMR and LC-MS/MS. Purity was > 95%, as determined by HPLC [27 (link)].
Karotinocyte sfm medium
Manufactured by Thermo Fisher Scientific
Keratinocyte SFM medium is a serum-free, low-calcium medium designed for the culture of human epidermal keratinocytes. The medium supports the growth and maintenance of keratinocytes without the need for serum or feeder cells.
Automatically generated - may contain errors
2 protocols using karotinocyte sfm medium
1
Characterization of Enzalutamide-Resistant Prostate Cell Lines
2
Characterization of Enzalutamide-Resistant Prostate Cell Lines
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C4–2B MDVR (C4–2B Enzalutamide resistant) and CWR22Rv1 cells were maintained in RPMI1640, whereas HEK293 and IMR90 fibroblast cells were cultured in DMEM supplemented with 10% fetal bovine serum (FBS), 100 units/ml penicillin, and 0.1 mg/ml streptomycin. RWPE-1 cells were grown in Karotinocyte SFM medium (Gibco). All cell line experiments were performed within six months of receipt from the ATCC or resuscitation after cryopreservation. C4–2B cells were kindly provided and authenticated by Dr. Leland Chung Lab at Cedars-Sinai Medical Center (Los Angeles, CA, USA). C4–2B MDVR cells were maintained in medium containing 20 μM Enzalutamide. Parental C4–2B cells were passaged alongside the resistant cells as an appropriate control [25 (link),26 ]. All cell lines were routinely tested as mycoplasma-free by PCR and authenticated using the short tandem repeat (STR) method. All cells were maintained at 37 °C in a humidified incubator with 5% carbon dioxide. Enzalutamide, apalutamide, darolutamide, and abiraterone acetate were purchased from Selleck Chemical. JG98 and JG231 were synthesized as described and their identities confirmed by 1H NMR and LC-MS/MS. Purity was > 95%, as determined by HPLC [27 (link)].
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