Male C57BL/6 mice (Charles River Laboratories) were purchased from the Animal Breeding Centre of Beijing Vital River Laboratories Company (Beijing, China). All animals were housed at 22 ± 2°C with a relative humidity of 50 ± 10% and a 12 h light/12 h dark cycle. The animals had free access to water and fodder (Beijing Keaoxieli Co, Ltd.). All experimental animal procedures were approved in accordance with the Guide for the Care and Use of Laboratory Animals published by the US National Institutes of Health (NIH publication No. 85-23, revised 1996) and were approved by the Academy of Chinese Medical Science's Administrative Panel on Laboratory Animal Care.
The concatenated tandem array of consensus transcription factor response element (catTFRE) DNA was synthesized by GenScript (Piscataway, NJ, USA). Biotinylated catTFRE primers were synthesized by Sigma (St. Louis, MO USA). Nuclear extract prep kits were purchased from Thermo Fisher. DHI (batch number: 13011023) was obtained from Shandong Danhong Pharmaceutical Co., Ltd. (Shandong, China). BNC (batch number: Z131117) was provided by the Buchang Pharma Co., Ltd. (Shanxi, China). Deionized water (R > 18.2 MΩ) used for all experiments was purified by using a Millipore purification system (Billerica, MA, USA). All other chemicals were of analytical grade reagent unless stated otherwise.
The concatenated tandem array of consensus transcription factor response element (catTFRE) DNA was synthesized by GenScript (Piscataway, NJ, USA). Biotinylated catTFRE primers were synthesized by Sigma (St. Louis, MO USA). Nuclear extract prep kits were purchased from Thermo Fisher. DHI (batch number: 13011023) was obtained from Shandong Danhong Pharmaceutical Co., Ltd. (Shandong, China). BNC (batch number: Z131117) was provided by the Buchang Pharma Co., Ltd. (Shanxi, China). Deionized water (R > 18.2 MΩ) used for all experiments was purified by using a Millipore purification system (Billerica, MA, USA). All other chemicals were of analytical grade reagent unless stated otherwise.