Donepezil hydrochloride

The fused silica capillary, with dimensions of 75 μm i.d. (375 μm o.d.) × 60.2 cm (50.0 cm to detection window), was purchased from Yongnian Reafine Chromatogram Equipment Co., Ltd. (Yongnian, China) and used for IMER fabrication and CE separation. AChE (220 U/mg), acetylthiocholine iodide (ATCh, ≥98.0%), and donepezil hydrochloride (≥98.0%) were all purchased from Macklin Biochemical Technology Ltd. Co. (Shanghai, China). Bovine serum albumin (BSA, ≥98.0%) was purchased from Aladdin Reagents (Shanghai) Co., Ltd. (Shanghai, China). Hexadimethrine bromide (HDB, ≥94.0%) was obtained from Sigma-Aladdin Chemical Co. (Shanghai, China). Concanavalin A (Con A) was from Baisha Biotechnology Co. (Anhui, China). Salvianolic acid B and Gastrodin were obtained from Shanghai Yuanye Biotechnology Co. (Shanghai, China). All the reagents were of analytical grade, and deionized water was used throughout all the experiments. All solutions were prepared in deionized (DI) water. All the solutions were stored in the refrigerator at 4 °C and filtered with 0.45 μm pore membrane filters prior to use.

The 6 raw herbs of DSS were purchased from Kangmei Pharmaceutical Limited Company (Guangzhou, China). Donepezil hydrochloride (D849374) and scopolamine hydrobromide trihydrate (S860151) were from Macklin (Shanghai, China); FITC-dextran (^#46944) was from Sigma system (ChemiDoc MP, Bio-Rad, California, USA); total cholesterol Assay Kit (A111-1-1) and Triglyceride Assay Kit (A110-1-1) were from Nanjing Jiancheng; and Malondialdehyde (MDA) ELISA Kit (JL13339), Adiponectin (ADPN) Kit (JL20696D), Low-Density Lipoprotein Cholesterol (LDL-C) Kit (JL20313), High-Density Lipoprotein Cholesterol (HDL-C) Kit (JL20356), Tumour Necrosis Factor-α Kit (TNF-α) (JL10484) and Interleukin 6 (IL-6) Kit (JL20313) were from Jiang Lai Biological (Shanghai, China). Antibodies included rabbit anti-ZO-1 (PB9234, Boster, Pleasanton, CA, USA), rabbit anti-OCLN (A01246-2, Boster), rabbit anti-OCLN (#91131, CST, Danvers, MA, USA) in brain immunofluorescence, rabbit anti-ZO-1 (#13663, CST) in brain immunofluorescence, rabbit anti-PPAR-γ (^#2435, CST), Cy3-labelled Goat Anti-Rabbit IgG (H+L) (A0516, Beyotime, Shanghai, China), rabbit anti-LXR alpha+beta (ab21669, Abcam, Cambridge, MA, USA), rabbit anti-actin (^#2118, CST), and rabbit anti-GAPDH (AP0063, Bioworld, St. Louis Park, MN, USA).