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2 protocols using penicillin streptomycin p s double antibody

1

Optimizing Cell Culture Conditions

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Phosphate-buffered saline (PBS), Trypsin/EDTA solution, Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and penicillin/streptomycin (P/S) double antibody were purchased from Gibco. Accutase® solution and NaAsO2 were purchased from Sigma-Aldrich. Endothelial cell medium (ECM) was purchased from ScienCell Research Laboratories. Primary antibodies (anti-vimentin and anti-E-cadherin) were purchased from Cell Signaling Technologies. Additional primary antibodies (anti-CD34, anti-N-cadherin) were purchased from Abcam. A Cell Counting Kit-8 (CCK-8) was purchased from DOJINDO. Zn(NO3)2·6H2O, 2-methylimidazole, polyethylene glycol (2000), indocyanine green (ICG) and fluorescein isothiocyanate (FITC) were purchased from Aladdin. Methanol was purchased from Guangzhou Chemical Reagent Factory. An Annexin V-FITC/PI apoptosis detection kit was purchased from BD Biosciences. A calcein-AM/PI double staining kit was purchased from Solarbio, Beijing.
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2

Optimizing Cell Culture and Viability Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
Phosphate-buffered saline (PBS), Trypsin/EDTA solution, Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), and penicillin/streptomycin (P/S) double antibody were purchased from Gibco. Accutase® solution and NaAsO 2 were purchased from Sigma-Aldrich. Endothelial cell medium (ECM) was purchased from ScienCell Research Laboratories. Primary antibodies (anti-vimentin and anti-E-cadherin) were purchased from Cell Signaling Technologies. Additional Primary antibodies (anti-CD34, anti-N-cadherin) were purchased from Abcam. A Cell Counting Kit-8 (CCK-8) was purchased from DOJINDO. Zn(NO 3 ) 2 •6H 2 O, 2-methylimidazole, polyethylene glycol (2000), indocyanine green (ICG) and fluorescein isothiocyanate (FITC) were purchased from Aladdin. Methanol was purchased from Guangzhou Chemical Reagent Factory. An Annexin V-FITC/PI apoptosis detection kit was purchased from BD Biosciences. A calcein-AM/PI double staining kit was purchased from Solarbio, Beijing.
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