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Phospho tbk1 nak ser172

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Phospho-TBK1/NAK (Ser172) is a lab equipment product that detects phosphorylation of TBK1/NAK at serine 172. It is used to monitor the activation state of this kinase.

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Lab products found in correlation

Hrp conjugated anti rabbit and anti mouse secondary antibodies, by Boster Bio (2 mentions) Lipopolysaccharides (lps), by Merck Group (2 mentions) Ripk3, by Cell Signaling Technology (2 mentions) Nf κb, by Proteintech (2 mentions) Ab178945, by Wuhan Servicebio Technology (2 mentions) Gapdh, by Wuhan Servicebio Technology (2 mentions) Ab183218, by Abcam (2 mentions) Ab40676, by Abcam (2 mentions) Ab168352, by Abcam (2 mentions) Cd163, by BioLegend (2 mentions) Phospho nf κb, by Cell Signaling Technology (2 mentions) M csf, by BioLegend (2 mentions) Phospho ripk3, by Cell Signaling Technology (2 mentions) F4 80, by BioLegend (2 mentions) Middlebrook 7h10 agar, by BD (1 mentions) Middlebrook 7h9 broth, by BD (1 mentions) Monodansylcadaverine mdc, by Merck Group (1 mentions) Phospho 4e bp1 thr37 46, by Cell Signaling Technology (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)

Spelling variants of this product

Phospho-TBK1 (23 citations) Phospho-TBK1 (Ser172) (11 citations) TBK1/NAK (8 citations) Phospho-TBK1/NAK (6 citations) Anti-phospho-TBK1/NAK (Ser172) (D52C2) (2 citations)

4 protocols using phospho tbk1 nak ser172

1

Autophagy Modulation in Mycobacterial Infection

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The following reagents were used in this study: Dexamethasone (Sigma-Aldrich, D4902), Hydrocortisone (Sangon Biotech, A610506), Monodansylcadaverine (MDC, Sigma-Aldrich, D4008), Rapamycin (Sigma-Aldrich, V900930), DMSO (Sigma-Aldrich, D2650), Bafilomycin A1 (Santa Cruz, sc-201550), Texas-red (Invitrogen, T-6134), TBK1 inhibitor BX795 (Selleckchem, S1274), Trizol reagent (Invitrogen, 15596-018), Middlebrook 7H9 Broth (BD Difco Laboratories, 271310), Middlebrook 7H10 Agar (BD Difco Laboratories, 262710). The following antibodies were used in this study: LC3 (Novus Biologicals, NB100-2220), GAPDH (ZSGB-BIO, TA-08), CD63 (Santa cruz, sc-15363), ATG5 (Cell Signaling, 12994), ATG6 (Cell Signaling, 3738), ATG7 (Cell Signaling, 8558), ATG12 (Cell Signaling, 4180), β-Tubulin (Cell Signaling, 2128), TBK1/NAK (Cell Signaling, 3013), Phospho-TBK1/NAK (Ser172) (Cell Signaling, 5483), Akt (Cell Signaling, 4691), Phospho-Akt (Ser473) (Cell Signaling, 4060), p70 S6 Kinase (Cell Signaling, 9202), Phospho-p70 S6 Kinase (Thr389) (Cell Signaling, 9206), 4E-BP1 (Cell Signaling, 9644), Phospho-4E-BP1 (Thr37/46) (Cell Signaling, 2855).
Wang J., Wang R., Wang H., Yang X., Yang J., Xiong W., Wen Q, & Ma L. (2017). Glucocorticoids Suppress Antimicrobial Autophagy and Nitric Oxide Production and Facilitate Mycobacterial Survival in Macrophages. Scientific Reports, 7, 982.
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2

Immunoblot Analysis of Tumor Signaling Pathways

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The proteins derived from 4T1 tumor tissues were extracted and further quantified by the BCA kit. Subsequently, the samples with equal amounts of proteins (20 μg) were separated through SDS-PAGE gel. Then the PVDF membranes with transferred proteins were blocked by 5% skim milk. After incubation with primary antibodies, including phospho-IRF-3 (Ser396) (4D4G) (4947 S, Cell Signaling Technology, 1:1000 dilution), IRF-3 (D83B9) (4302 S, Cell Signaling Technology, 1:1000 dilution), phospho-TBK1/NAK (Ser172) (D52C2) (5483 S, Cell Signaling Technology, 1:1000 dilution), TBK1/NAK (D1B4) (3504 T, Cell Signaling Technology, 1:1000 dilution), phospho-STING (Ser365) (D8F4W) (72971 S, Cell Signaling Technology, 1:1000 dilution), STING (A21051, ABclonal, 1:10000 dilution), and Tubulin β (bs-20694R, Bioss, 1:1000 dilution) overnight at 4 °C, the PVDF films were incubated with anti-rabbit IgG, HRP-linked antibody (7074 S, Cell Signaling Technology, 1:2000 dilution) for another 1 h. Chemiluminescence detection was carried out for protein band visualization with ECL Substrate. Uncropped and unprocessed full scan images of all western blots can be found in the Source Data file.
Wang K., Li Y., Wang X., Zhang Z., Cao L., Fan X., Wan B., Liu F., Zhang X., He Z., Zhou Y., Wang D., Sun J, & Chen X. (2023). Gas therapy potentiates aggregation-induced emission luminogen-based photoimmunotherapy of poorly immunogenic tumors through cGAS-STING pathway activation. Nature Communications, 14, 2950.
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3

Immune Signaling Pathway Profiling

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We used antibodies against TBK1 (Abcam, ab40676, 1:1000), phospho-TBK1/NAK (Ser172) (Cell Signaling, #5483, 1:1000), RIPK3 (Cell Signaling, #95702, 1:1000), phospho-RIPK3 (Cell Signaling, #91702, 1:1000), NF-κB (Proteintech, 10745-1-AP, 1:1000), phospho-NF-κB (Cell Signaling, #3033, 1:1000), GLUD1 (Abcam, ab168352, 1:1000), TNF-α (Abcam, ab183218, 1:1000), iNOS (Abcam, ab178945, 1:1000), GAPDH (Servicebio, GB12002, 1:1000). CD86 (Biolegend,105007), CD163 (Biolegend, 333606), and F4/80 (Biolegend, 123109). HRP-conjugated anti-rabbit and anti-mouse secondary antibodies were obtained from Boster Biological Technology (Wuhan, China). LPS was purchased from Sigma (L2880). Neutral liposomes and control liposomes (F70101C-NC-2) were purchased from FormuMax. M-CSF (576402) was obtained from Biolegend.
Pan L., Yang L., Yi Z., Zhang W, & Gong J. (2022). TBK1 participates in glutaminolysis by mediating the phosphorylation of RIPK3 to promote endotoxin tolerance. Molecular immunology, 147.
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4

Immune Signaling Pathway Profiling

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We used antibodies against TBK1 (Abcam, ab40676, 1:1000), phospho-TBK1/NAK (Ser172) (Cell Signaling, #5483, 1:1000), RIPK3 (Cell Signaling, #95702, 1:1000), phospho-RIPK3 (Cell Signaling, #91702, 1:1000), NF-κB (Proteintech, 10745-1-AP, 1:1000), phospho-NF-κB (Cell Signaling, #3033, 1:1000), GLUD1 (Abcam, ab168352, 1:1000), TNF-α (Abcam, ab183218, 1:1000), iNOS (Abcam, ab178945, 1:1000), GAPDH (Servicebio, GB12002, 1:1000). CD86 (Biolegend,105007), CD163 (Biolegend, 333606), and F4/80 (Biolegend, 123109). HRP-conjugated anti-rabbit and anti-mouse secondary antibodies were obtained from Boster Biological Technology (Wuhan, China). LPS was purchased from Sigma (L2880). Neutral liposomes and control liposomes (F70101C-NC-2) were purchased from FormuMax. M-CSF (576402) was obtained from Biolegend.
Pan L., Yang L., Yi Z., Zhang W., & Gong J. (2022). TBK1 Participates in Glutaminolysis by Mediating the Phosphorylation of RIPK3 to Promote Endotoxin Tolerance.
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