Lkb ultrascan xl laser densitometer

Manufactured by Kodak

Sourced in United Kingdom

The LKB Ultrascan XL Laser Densitometer is a lab equipment designed for the quantitative analysis of electrophoretic gels and blots. It uses a laser-based scanning system to precisely measure the optical density of samples, providing accurate and reproducible data for researchers.

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Lab products found in correlation

Ecl plus kit, by Cytiva (2 mentions) Rabbit anti mmp3, by Abcam (1 mentions) Nitrocellulose, by Bio-Rad (1 mentions) Horseradish peroxidase conjugated goat anti mouse antibody, by Agilent Technologies (1 mentions)

2 protocols using lkb ultrascan xl laser densitometer

Western Blotting of MMP-3 and MMP-12

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Western blotting was performed according to a modified method previously described (19 (link)). A rabbit anti-MMP-3 (10 ng/mL; Abcam, Cambridge, UK) and a sheep anti-MMP-12 (10 ng/mL; Abcam) were used as primary antibodies. Appropriate horseradish peroxidase-conjugated antibodies (DAKO, High Wycombe, UK) were used as secondary antibodies, and the reaction was developed with the ECL plus kit (Amersham Biosciences, Little Chalfont, UK). When required, blots were stripped and analyzed for internal loading controls, and bands were quantified using an LKB Ultrascan XL Laser Densitometer (Kodak, Hemel Hempstead, UK).

Lenti M.V., Facciotti F., Miceli E., Vanoli A., Fornasa G., Lahner E., Spadoni I., Giuffrida P., Arpa G., Pasini A., Rovedatti L., Caprioli F., Travelli C., Lattanzi G., Conti L., Klersy C., Vecchi M., Paulli M., Annibale B., Corazza G.R., Rescigno M, & Di Sabatino A. (2022). Mucosal Overexpression of Thymic Stromal Lymphopoietin and Proinflammatory Cytokines in Patients With Autoimmune Atrophic Gastritis. Clinical and Translational Gastroenterology, 13(7), e00510.

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Quantitative Immunoblotting Analysis of IFN-α

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Immunoblotting was performed according to a modified method described previously [17] . Proteins (100 g) from 24 hcultured duodenal biopsies were loaded and subjected to 10% SDS-PAGE under reducing conditions, followed by nitrocellulose (Bio-Rad Laboratories, Hercules, California, USA) transfer. Mouse anti-human IFN-␣ (1:1000 dilution; Proteintech, Manchester, UK) was used as primary antibody. Horseradish peroxidase-conjugated goat anti-mouse antibody (1:2000 dilution; DAKO, High Wycombe, UK) was then used as secondary antibody, and the reaction was developed with the ECL plus kit (Amersham Biosciences, Little Chalfont, UK). Blots were stripped and analyzed for internal loading control using rabbit anti-␤-actin antibodies (1:5000 dilution; Abcam, Cambridge, UK). Bands were quantified using an LKB Ultrascan XL Laser Densitometer (Kodak, Hemel Hempstead, UK).

Di Sabatino A., Giuffrida P., Fornasa G., Salvatore C., Vanoli A., Naviglio S., De Leo L., Pasini A., De Amici M., Alvisi C., Not T., Rescigno M, & Corazza G.R. (2016). Innate and adaptive immunity in self-reported nonceliac gluten sensitivity versus celiac disease. Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver, 48(7).

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