Human liver microsomes hlm

Manufactured by Corning

Sourced in United States

Human liver microsomes (HLM) are an in vitro test system derived from human liver tissue. HLM contain the metabolic enzymes found in the endoplasmic reticulum of hepatocytes and can be used to study the biotransformation of drugs and other xenobiotics.

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Lab products found in correlation

Synergy uv system, by Merck Group (1 mentions) Magnesium chloride, by Merck Group (1 mentions) Nicotinamide adenine dinucleotide phosphate, by Merck Group (1 mentions) S 5 adenosyl l methionine, by Merck Group (1 mentions) L glutathione reduced, by Merck Group (1 mentions) Gallic acid, by Merck Group (1 mentions) Acetic acid, by Merck Group (1 mentions) Glucose 6 phosphate (g6p), by Merck Group (1 mentions) Glucose 6 phosphate dehydrogenase, by Merck Group (1 mentions) 1 hydroxy midazolam 1 oh mdz, by Merck Group (1 mentions)

2 protocols using human liver microsomes hlm

Enzymatic catechin metabolism analysis

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Green tea catechins [(+)catechin (C), (−)epicatechin (EC), (−)catechin gallate (CG), (−)epicatechin gallate (ECG), (−)gallocatechin (GC), (−)epigallocatechin (EGC), (−)epigallocatechin gallate (EGCG), (−)gallocatechin gallate (GCG)], synthetic gallate esters [ethyl gallate (EG), propyl gallate (PG), butyl gallate (BG), octyl gallate (OG) and lauryl gallate (LG)], gallic acid (GA), reduced L-glutathione (GSH), S-(5′-adenosyl)-L-methionine (SAM), nicotinamide adenine dinucleotide phosphate (NADP⁺), glucose-6-phosphate, magnesium chloride and glucose-6-phosphate dehydrogenase, potassium (mono- and di-basic) phosphate, in addition to HPLC-grade acetonitrile (ACN), methanol, and acetic acid were purchased from Sigma-Aldrich (Oakville, ON, Canada). Human liver microsomes (HLM, pooled from 50 donors) were purchased from Corning (Corning, NY, USA). Ultrapure water was obtained from a Millipore Synergy UV system (Billerica, MA, USA).

Ousji O, & Sleno L. (2022). Structural Elucidation of Novel Stable and Reactive Metabolites of Green Tea Catechins and Alkyl Gallates by LC-MS/MS. Antioxidants, 11(9), 1635.

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LC-MS/MS Analysis of Hepatic Metabolism

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All the solvents used for LC-MS/MS were obtained from Honeywell (B&J, AC/HPLC-certified solvents) and were of analytical grade. DTZ hydrochloride, midazolam (MDZ), and 1-hydroxy midazolam (1-OH MDZ) were obtained from Sigma-Aldrich. ERY, NDD, NDE, and NV were purchased from Toronto Research Chemicals. TAO was obtained from Enzo Life Sciences. Pooled (n = 35 livers) human-liver microsomes (HLM) and NADPH solution A and solution B were obtained from Corning Life Sciences.

Yadav J., Korzekwa K, & Nagar S. (2018). Improved Predictions of Drug–Drug Interactions Mediated by Time-Dependent Inhibition of CYP3A. Molecular pharmaceutics, 15(5), 1979-1995.

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