The urine samples were collected on admission to critical care areas (day 1). The urine was frozen at −80 °C within the first 30 min after sample collection. The urinary concentrations of TIMP-2 and IGFBP7 were determined using commercially available ELISA kits (Human TIMP-2 Quantikine ELISA Kit, R&D, Minneapolis, Minnesota; Human IGFBP7 ELISA Kit, Abcam, Cambridge, UK) following manual instructions. The ELISA plates were read at O.D. of 450 and the calculations were done according to the signal that was given by the standard curve of each kit. NGAL determinations were done using the NGAL kit (Abbott, Chicago, IL, USA) according to the manual instructions and using the Abbott™ ARCHITECT™ Analyzer.
Ngal kit
Manufactured by Abbott
Sourced in United States
The NGAL kit is a laboratory diagnostic tool used to measure the levels of the NGAL (Neutrophil Gelatinase-Associated Lipocalin) biomarker. NGAL is a protein that can serve as an indicator of kidney function. The kit provides a quantitative assessment of NGAL concentrations in biological samples, such as urine or plasma, to support healthcare professionals in evaluating kidney health.
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2 protocols using ngal kit
1
Urinary Biomarkers in Critical Care
2
Urinary Biomarkers for Acute Kidney Injury
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The urine samples were collected on day 1 and on day 5. The urine was frozen at −80 °C within the first 30 min after sample collection. The urinary concentrations of TIMP-2 and IGFBP7 were determined using commercially available ELISA kits (Human TIMP-2 Quantikine ELISA Kit, R&D, Minneapolis, Minnesota, USA; Human IGFBP7 ELISA Kit, Abcam, Cambridge, UK) following manual instructions. The ELISA plates were read at O.D. of 450 and the calculations were done according to the signal that was given by the standard curve of each kit. NGAL determinations were done using the NGAL kit (Abbott, Chicago, IL, USA) according to the manual instructions and using the Abbott ™ ARCHITECT™ Analyzer (Chicago, IL, USA).
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