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Mouse ctx 1 elisa kit

Manufactured by Immunodiagnostic Systems

The Mouse CTX-I ELISA kit is a sandwich enzyme-linked immunosorbent assay (ELISA) designed for the quantitative measurement of mouse C-terminal telopeptide of type I collagen (CTX-I) in serum, plasma, or cell culture supernatants.

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2 protocols using mouse ctx 1 elisa kit

1

Quantifying Cytokine and Bone Markers

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Mouse high-sensitivity IFN-α ELISA kit (42115-1) and IFN-β ELISA kit (42410-1) were purchased from PBL Assay Science. Mouse CTX-I ELISA kit (AC-06F1) and mouse PINP ELISA kit (AC-33F1) were purchased from Immunodiagnostic Systems. ELISA was performed using culture medium, serum, and bone marrow lysates. Serum was obtained from whole blood that was collected from a submandibular vein via facial vein puncture, coagulated for 30 min at room temperature, followed by centrifugation (2000 × g for 10 min, 4 °C) and collection of the supernatant (serum). Bone marrow was homogenized in a lysis buffer containing protease inhibitors at 4 °C for 30 min. ELISA was conducted in accordance with the manufacturer’s instructions. A standard curve was performed for each experiment.
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2

Measuring Cytokines and Bone Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse high-sensitivity IFN-α ELISA kit (42115-1) and IFN-β ELISA kit (42410-1) were purchased from PBL Assay Science. Mouse CTX-I ELISA kit (AC-06F1) and mouse PINP ELISA kit (AC-33F1) were purchased from Immunodiagnostic Systems. ELISA was performed using culture medium, serum, and bone marrow lysates. Serum was obtained from whole blood that was collected from a submandibular vein via facial vein puncture, coagulated for 30 minutes at room temperature, followed by centrifugation (2,000 × g for 10 min, 4°C) and collection of the supernatant (serum). Bone marrow was homogenized in a lysis buffer containing protease inhibitors at 4°C for 30 minutes. ELISA was conducted in accordance with the manufacturer's instructions. A standard curve was performed for each experiment.
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