For confocal microscopy, cells were grown on coverslips and then stained with anti-NRF2 (NRF2 C-20 SC722, Santa Cruz Biotechnology, Santa Cruz, CA, USA). Immediately before examination, cells were counterstained with DAPI (D1306, ThermoFisher Scientific, Grand Island, NY). Before experiments, cells were seeded on 24 mm glass coverslip for 24 h before microscopic analysis (Leica DMR, Heidelberg, Germany). The confocal microscope was equipped with a 488 nm Argon laser, 561-nm diode-pumped solid-state laser and a 405-nm ultraviolet laser. Images were acquired using an oil ×40 Plan-NEOFLUAR objective (1.3 NA) and an oil ×63 Plan-APOCHROMAT objective (1.4 NA).
Nrf2 c 20 sc 722
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Nrf2 (C-20) (SC-722) is a primary antibody produced by Santa Cruz Biotechnology. It is used to detect the Nrf2 (Nuclear factor erythroid 2-related factor 2) protein by researchers in various applications.
Automatically generated - may contain errors
Lab products found in correlation
Nf κb p65 sc 372, by Santa Cruz Biotechnology (2 mentions)
Corm 2 288144, by Merck Group (2 mentions)
N acetyl cysteine a7250 10g, by Merck Group (2 mentions)
Ferrous chloride fecl 2, by Sinopharm (2 mentions)
Diamidino 2 phenylindole dihydrochloride dapi c10005, by Beyotime (2 mentions)
Reactive oxygen species assay kit s0033, by Beyotime (2 mentions)
Annexin 5 fitc apoptosis detection kit c1062, by Beyotime (2 mentions)
Anti nqo1 antibody ab2346, by Abcam (2 mentions)
Corm 2, by Merck Group (2 mentions)
D1306, by Thermo Fisher Scientific (1 mentions)
3 protocols using nrf2 c 20 sc 722
1
Confocal Microscopy of NRF2 Staining
2
CORM-2 and NAC Modulate Stem Cell Behavior
Check if the same lab product or an alternative is used in the 5 most similar protocols
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CORM-2 (288144) and N-acetyl-cysteine (NAC) (A7250-10G) were purchased from Sigma-Aldrich (St.
Louis, MO, USA).CORM-2 was dissolved in dimethyl sulfoxide, and then diluted in culture media to achieve the required concentrations. Inactivated CORM-2 (iCORM-2) was prepared by dissolving CORM-2 under the same conditions for 3 days at room temperature to liberate all CO from the molecule [14]. NSC C17.2, a stable, fully characterized, mouse stem cell line [15] , was kindly provided by Prof. Jin WL (Institute of Nano Biomedicine and Engineering, Shanghai Jiao Tong University, Shanghai, China). Ferrous chloride (FeCl 2 ) was purchased from Sinopharm Chemical Reagent (Shanghai, China). Annexin V-FITC Apoptosis Detection Kit (C1062), Diamidino-2-phenylindole dihydrochloride (DAPI) (C10005) and Reactive Oxygen Species Assay Kit (S0033) were from Beyotime Biotechnology (Shanghai, China). The primary antibodies Nrf2 (C-20) (SC-722) and NF-κB P65 (SC-372) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
Anti-NQO1 antibody (ab2346) was from Abcam (Cambridge, UK). ß-Actin (4970),Tublin(2148) and H3 (12167), were from Cell Signaling Technology (USA).
Louis, MO, USA).CORM-2 was dissolved in dimethyl sulfoxide, and then diluted in culture media to achieve the required concentrations. Inactivated CORM-2 (iCORM-2) was prepared by dissolving CORM-2 under the same conditions for 3 days at room temperature to liberate all CO from the molecule [14]. NSC C17.2, a stable, fully characterized, mouse stem cell line [15] , was kindly provided by Prof. Jin WL (Institute of Nano Biomedicine and Engineering, Shanghai Jiao Tong University, Shanghai, China). Ferrous chloride (FeCl 2 ) was purchased from Sinopharm Chemical Reagent (Shanghai, China). Annexin V-FITC Apoptosis Detection Kit (C1062), Diamidino-2-phenylindole dihydrochloride (DAPI) (C10005) and Reactive Oxygen Species Assay Kit (S0033) were from Beyotime Biotechnology (Shanghai, China). The primary antibodies Nrf2 (C-20) (SC-722) and NF-κB P65 (SC-372) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
Anti-NQO1 antibody (ab2346) was from Abcam (Cambridge, UK). ß-Actin (4970),Tublin(2148) and H3 (12167), were from Cell Signaling Technology (USA).
3
CORM-2 and NAC Modulate Stem Cell Behavior
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CORM-2 (288144) and N-acetyl-cysteine (NAC) (A7250-10G) were purchased from Sigma-Aldrich (St.
Louis, MO, USA).CORM-2 was dissolved in dimethyl sulfoxide, and then diluted in culture media to achieve the required concentrations. Inactivated CORM-2 (iCORM-2) was prepared by dissolving CORM-2 under the same conditions for 3 days at room temperature to liberate all CO from the molecule [14]. NSC C17.2, a stable, fully characterized, mouse stem cell line [15] , was kindly provided by Prof. Jin WL (Institute of Nano Biomedicine and Engineering, Shanghai Jiao Tong University, Shanghai, China). Ferrous chloride (FeCl 2 ) was purchased from Sinopharm Chemical Reagent (Shanghai, China). Annexin V-FITC Apoptosis Detection Kit (C1062), Diamidino-2-phenylindole dihydrochloride (DAPI) (C10005) and Reactive Oxygen Species Assay Kit (S0033) were from Beyotime Biotechnology (Shanghai, China). The primary antibodies Nrf2 (C-20) (SC-722) and NF-κB P65 (SC-372) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
Anti-NQO1 antibody (ab2346) was from Abcam (Cambridge, UK). ß-Actin (4970),Tublin(2148) and H3 (12167), were from Cell Signaling Technology (USA).
Louis, MO, USA).CORM-2 was dissolved in dimethyl sulfoxide, and then diluted in culture media to achieve the required concentrations. Inactivated CORM-2 (iCORM-2) was prepared by dissolving CORM-2 under the same conditions for 3 days at room temperature to liberate all CO from the molecule [14]. NSC C17.2, a stable, fully characterized, mouse stem cell line [15] , was kindly provided by Prof. Jin WL (Institute of Nano Biomedicine and Engineering, Shanghai Jiao Tong University, Shanghai, China). Ferrous chloride (FeCl 2 ) was purchased from Sinopharm Chemical Reagent (Shanghai, China). Annexin V-FITC Apoptosis Detection Kit (C1062), Diamidino-2-phenylindole dihydrochloride (DAPI) (C10005) and Reactive Oxygen Species Assay Kit (S0033) were from Beyotime Biotechnology (Shanghai, China). The primary antibodies Nrf2 (C-20) (SC-722) and NF-κB P65 (SC-372) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
Anti-NQO1 antibody (ab2346) was from Abcam (Cambridge, UK). ß-Actin (4970),Tublin(2148) and H3 (12167), were from Cell Signaling Technology (USA).
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