Dribulose 1 5 diphosphate carboxylase

Deionized (18.2 MΩ) filtered water (0.22 μm) came from a Milli-Q system, Millipore SAS (Molsheim, France). Methanol, Folin Ciocalteu's reagent, sodium carbonate, sodium bicarbonate, sodium sulfite, sodium chloride, sodium hydrogen phosphate, sodium dihydrogen phosphate, Dribulose 1,5-diphosphate carboxylase (Rubisco) from spinach, 1,4-dithioerythritol (DTE), HCl 37%, pyrocatechol, tyrosinase from mushroom, and gallic acid, were purchased from Sigma-Aldrich (Darmstadt, Germany). Sodium dodecyl sulfate (SDS), ethylenediaminetetraacetic acid (EDTA) and trisaminomethan (Trizma Base) from Merck (Germany), coomassie brilliant blue G-250 (Serva, Heidelberg, Germany), glycin (Applichem, Darmstadt, Germany), Precision Plus Protein Kaleidoscope Prestained Protein Standards (Bio-Rad), PageRuler Plus, and Criterion Tris-HCl gels were from Bio-Rad (Hercules, USA).

Ultracentrifuged juices were filtered through Mini-UniPrep TM filters with pore size 0.2 μm (Whatman TM , GE Healthcare Life Sciences) before injection into a HPLC (Agilent, United States) with a Yarra 1.8 µm SEC-X150 column (size: 150 x 4.6 mm) (Phenomenex, United States). Four μL of juices were injected into the HPLC and compared to a D-Ribulose 1,5-diphosphate carboxylase (Rubisco) standard from spinach (Sigma-Aldrich, Germany). Protein was eluted with a 0.05 M PO4 buffer with 0.3 M NaCl and pH 6.8 at a flow of 0.3 mL min -1 . UV absorbance was measured at 214 nm. Size exclusion analysis was performed in triplicate.

Deionized (18.2 MΩ) filtered water (0.22 μm) came from a Milli-Q system, Millipore SAS (Molsheim, France).
L-leucine, methanol, Folin Ciocalteu's reagent, sodium carbonate, sodium bicarbonate, sodium sulphite, sodium chloride, sodium hydrogen phosphate, sodium dihydrogen phosphate, D-ribulose 1,5-diphosphate carboxylase (rubisco) from spinach, pyronin Y, 1,4-dithioerythritol (DTE), HCl 37%, L-3,4-dihydroxyphenylalanine, gallic acid, bovine serum albumin, sodium tetraborate, pepsin, pancreatin, and fluorescamine were purchased from Sigma-Aldrich (Darmstadt, Germany). Sodium dodecyl sulfate (SDS), trisaminomethan (Trizma Base), coomassie brilliant blue G-250 (Serva, Heidelberg, Germany), glycin (Applichem, Darmstadt, Germany), Spectra Multicolour Board range protein Ladder, Thermo 26634 (Thermo Scientific, Rockford, USA), Criterion Tris-HCl gels (Bio-Rad, Hercules, USA), trichloroacetic acid (TCA), and dried acetone were obtained from Merck, Germany.