N nervonoyl d erythro sphingosine

N-palmitoyl-D-erythro-sphingosine (Cer16:0), N-stearoyl-D-erythro-sphingosine (Cer18:0), N-lignoceroyl-D-erythro-sphingosine (Cer24:0) and N-nervonoyl-D-erythro-sphingosine (Cer24:1) were obtained from Avanti Polar Lipids, Inc., Alabaster, AL, while their respective isotope-labeled standards used as an internal standard (IS) were purchased as a mixture (LIPIDOMIX^® Mass Spec Standard solution) from Avanti Polar Lipids, Inc., Alabaster, AL, USA.

1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC), N-lignoceroyl-D-erythro-sphingosylphosphorylcholine (24:0 SM, lSM), N-nervonoyl-D-erythro-sphingosyl-phosphorylcholine (24:1 SM, nSM), N-lignoceroyl-D-erythro-sphingosine (24:0 Cer, lCer), N-nervonoyl-D-erythro-sphingosine (24:1 Cer, nCer), cholesterol (Chol), and the lipophilic fluorescent probe 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (Rho-PE) were purchased from Avanti Polar Lipids (Alabaster, AL). DiI (1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindocarbocyanine Perchlorate ('DiI'; DiIC18(3))) was supplied by Thermo Fisher (Waltham, MA, USA). Methanol and chloroform were from Fisher (Suwanee, GA). Buffer solution, unless otherwise stated, was 20 mM PIPES, 1 mM EDTA, 150 mM NaCl, pH 7.4. All salts and organic solvents were of analytical grade.