Anti ucp1 u6382

For Western blotting, we used anti-UCP1 (U6382, 1:1000) and anti–β-Actin (A3854, 1:40,000) from MilliporeSigma; anti-PGC1α (ab54481, 1:1000), anti-FGF21 (ab64857, 1:1000) from Abcam; anti-C/EBPβ (sc7962, 1:1000) from Santa Cruz Biotechnology; and anti-Nr1d1 (Rev-Erbα) (13418, 1:1000), anti–Tyrosine Hydroxylase (2792, 1:1000), and anti-Phospho-(Ser/Thr) PKA Substrate (9621, 1:1000) from Cell Signaling Technology. For flow cytometry, we used anti-F4/80 (123110, 1:200), anti-CD206 (141704, 1:200), anti-CD11b (101228, 1:200), and anti-CD11c (117310, 1:200) from BioLegend.

Recombinant Murine BCA-1/BLC (CXCL13; 250-24), recombinant murine IL-4 (214–14), and recombinant murine M-CSF (315–02) were purchased from PeproTech Inc. (NJ, USA). Lipopolysaccharides (LPS, L2654), anti-β-actin (A3854), and anti-UCP1 (U6382) primary antibodies were obtained from Millipore Sigma Inc. (USA). Mouse anti-PRDM16 antibody (ab303534) was purchased from Abcam Inc. (USA). Mouse anti-CXCL13/BLC/BCA-1 antibody (AF470) and CXCL13 ELISA Kits (MCX130) were purchased from RDSystems Inc. (USA). Mouse adipose triglycerides lipase (ATGL, #2138), Acetyl-CoA Carboxylase (ACC, #3676), Phospho-Acetyl-CoA Carboxylase (Ser79, p-ACC, #3661), hormone-sensitive lipase (HSL, #4107) and Phospho-HSL (Ser565, p-HSL, #4137) were purchased from Cell Signaling Technology Inc. (USA). Anti-mouse TNFα primary antibody (A11534) was purchased from ABclonal Technology Co., Ltd. (China). Mouse TNFα ELISA Kits (CME0004) were purchased from 4A Biotech., Ltd (China). Flow cytometric antibodies including PE/cy7-F4/80 (#123114), APC-CD19 (#115512), PE-CD206 (#141705), FITC-CD45 (#103108), Pacific Blue-CD11b (#101224), PerCP/cy5.5-CD3 (#100328) and PerCP-CD11c (#117325) were purchased from Biolegend Inc. (USA).