Quercetin (≥98%) and sodium carboxymethyl cellulose (CMC) were purchased from Yuanye Biotechnology (Shanghai, China). DMSO was from Sigma-Aldrich (St. Louis, MO, USA). The RPMI 1640 medium and DMEM were from Hyclone (Logan, UT, USA). Fetal bovine serum was from Biological Industries (Kibbutz Beit-Haemek, Israel). Penicillin and streptomycin were from Beyotime (Shanghai, China). Trypsin and Annexin V-FITC cell apoptosis detection kit were from KeyGEN BioTECH (Nanjing, China). Cell Counting Kit-8 (CCK-8) was from Dojindo (Kumamoto, Japan). 3-Methyladenine (3-MA), hydroxychloroquine sulfate (HCQ), MK-2206 (MK), SC79 (SC), SP600125 (SP), PD0325901 (PD) and SB203580 (SB) were from Selleck Chemical (Houston, TX, USA).
Annexin 5 fitc cell apoptosis detection kit
Manufactured by Keygen Biotech
Sourced in China
The Annexin V-FITC cell apoptosis detection kit is a laboratory product that allows for the detection and quantification of apoptosis, a process of programmed cell death, in cell samples. The kit utilizes Annexin V, a protein that binds to phosphatidylserine, a molecule exposed on the surface of cells undergoing apoptosis, and FITC, a fluorescent dye, to enable the visualization and analysis of apoptotic cells.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Sartorius (1 mentions)
Streptomycin, by Beyotime (1 mentions)
Penicillin, by Beyotime (1 mentions)
Trypsin, by Keygen Biotech (1 mentions)
Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions)
Quercetin, by Yuanye Bio-Technology (1 mentions)
Dulbecco modified eagle medium (dmem), by Corning (1 mentions)
Novocyte, by Agilent Technologies (1 mentions)
Cell cycle detection kit, by Keygen Biotech (1 mentions)
Flow cytometry, by BD (1 mentions)
5 protocols using annexin 5 fitc cell apoptosis detection kit
1
Quercetin and Autophagy Modulators in Cancer
2
Annexin V-FITC Apoptosis Assay
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Apoptosis was determined using the Annexin V-FITC cell apoptosis detection kit (KeyGEN, Nanjing, China). Briefly, IPEC-J2 cells were cultured in 6-well plates for 16 h before treatment, pretreated with Ly294002, and then treated with RSV (0 μM, 25 μM, and 50 μM) or H2O2 according to the experimental design. Following each specific treatment, cells were collected, washed twice with ice-cold PBS, and then centrifuged at 2000 rpm for 5 min. Cells were resuspended in 500 μL of 1x binding buffer and transferred to sterile flow cytometry glass tubes. 5 μL of Annexin V-FITC and 5 μL of PI were added, and samples were incubated at room temperature (25°C) in dark conditions for 10 min. Finally, flow cytometric analysis was performed according to the manufacturer's instructions (Beckman, Fullerton, California, USA).
3
Apoptosis Quantification of ASMCs
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Apoptosis of ASMCs was detected by staining with the Annexin V-FITC cell apoptosis detection kit (Nanjing, keygen, Biotech Co., Ltd, China). Cells were harvested and incubated in diluted binding buffer, before adding 5 µL propidium iodide (PI, 20 µg/mL) for 15 min in the dark. Apoptosis was measured using a flow cytometer (BD, Franklin Lakes, NJ, USA).
4
Annexin V-FITC Apoptosis Assay
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Following transfection and/or treatment, cells were collected and digested with trypsin (trypsin) without ethylenediaminetetraacetic acid at room temperature for 1 min. The digestion was terminated by adding DMEM (Corning) containing 10% FBS. The cells were centrifuged at 1000 × g at room temperature for 3 min and the supernatant was removed. The cells were washed twice with pre-cooled PBS and resuspended in 1× Annexin V binding buffer. Following the protocols of the Annexin V-FITC cell apoptosis detection kit (KeyGen Biotech), cells were stained with 5 µl Annexin V-FITC and 5 µl propidium iodide (PI) and measured by flow cytometry (Novocyte, Agilent, USA). The apoptosis rate is a sum of early apoptosis (Annexin V positive/PI negative) and late apoptosis (Annexin V positive/ PI positive).
5
Apoptosis and Cell Cycle Analysis
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Apoptosis was detected using the Annexin V-FITC Cell Apoptosis Detection Kit (Keygen, Nanjing, China), and cell cycle was detected using the Cell Cycle Detection Kit (Keygen). Cell apoptosis and cycle were analyzed by Flow cytometry (BD, San Diego, CA, USA).
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