Nci hcc827 hcc827

Three human lung adenocarcinoma cell lines were used in the present study. PC-9 with an exon 19 in-frame deletion was provided from Immuno-Biological Laboratories (Gunma, Japan). NCI-HCC827 (HCC827) with a deletion in exon 19, A549 (EGFR wild type cell line) and BET2A (normal lung cell line) were purchased from the American Type Culture Collection (ATCC, Manassas, VA). PC-9 and HCC827 were cultured in RPMI1640 (Wako, Osaka, Japan) containing 10% fetal bovine serum (FBS; BioWest, Nuaille, France) and 1% penicillin and streptomycin (Wako Pure Chemical Industries, Osaka, Japan). These cell lines were obtained from 2010 through 2016, amplified and frozen, and one aliquot of each was thawed for this research. All cells were routinely screened for the absence of mycoplasma, and at least three independent experiments were performed for each condition.

Four human lung adenocarcinoma cell lines were used in this study. PC-9 with an exon 19 in-frame deletion was provided from Immuno-Biological Laboratories (Gunma, Japan). NCI-HCC827 (HCC827) with a deletion in exon 19, NCI-H1975 (L858R/T790M), and H1650 with a deletion in exon 19 and PTEN null were purchased from the American Type Culture Collection (Manassas, VA, USA). These cell lines were cultured in RPMI1640 (FUJIFILM Wako Pure Chemical Industries, Osaka, Japan), including 10% fetal bovine serum (BioWest, Nuaillé, France) and 1% penicillin and streptomycin (FUJIFILM Wako Pure Chemical Industries), at 37 °C in a 5% CO2 incubator as previously described [12 (link)]. All cells were constantly examined for the absence of mycoplasma, and each experiment was performed independently three times for each condition.