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4 protocols using paca2

1

Pancreatic Cancer Cell Culture and Treatments

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Human normal pancreatic cell line (H6C7) and PDAC cell lines (PANC-1, CFPAC1, BXPC3, PACA2, SW1990) were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco's modi ed Eagle's medium (DMEM, Gibco) supplemented with 10% FBS and 1% penicillin/streptomycin. All cells were incubated at 37°C in humidi ed air containing 5% CO2. Panobinostat(T2383), THZ1(T3664), Gemcitabine(T0251), (+)-JQ-1(T2110), cisplatin(T1564), 5-Fluorouracil(T0984) and terbina ne(T6702) were purchased from Target Mol (Shanghai, China).
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2

Culturing Pancreatic Cell Lines

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A normal human pancreatic cell line (HPDE) and six different PC cell lines (AsPC-1, Capan-2, SW1990, PANC-1, PaCa-2, and BxPC-3) were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China) and cultured in RPMI-1640 medium (Gibco) containing 10% fetal bovine serum (FBS, Gibco) in a humidi ed incubator containing 5% CO 2 at 37 °C. All cell lines were authenticated and tested to be mycoplasma-free.
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3

Culturing Pancreatic Cell Lines

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A normal human pancreatic cell line (HPDE) and six different PC cell lines (AsPC-1, Capan-2, SW1990, PANC-1, PaCa-2, and BxPC-3) were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China) and cultured in RPMI-1640 medium (Gibco) containing 10% fetal bovine serum (FBS, Gibco) in a humidi ed incubator containing 5% CO 2 at 37 °C. All cell lines were authenticated and tested to be mycoplasma-free.
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4

Pancreatic Cancer Cell Culture and Treatments

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Human normal pancreatic cell line (H6C7) and PDAC cell lines (PANC-1, CFPAC1, BXPC3, PACA2, SW1990) were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco's modi ed Eagle's medium (DMEM, Gibco) supplemented with 10% FBS and 1% penicillin/streptomycin. All cells were incubated at 37°C in humidi ed air containing 5% CO2. Panobinostat(T2383), THZ1(T3664), Gemcitabine(T0251), (+)-JQ-1(T2110), cisplatin(T1564), 5-Fluorouracil(T0984) and terbina ne(T6702) were purchased from Target Mol (Shanghai, China).
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