miR-125a-5p mimics (5′-AGUGUUCAAUCCCAGAGUCCCU-3′) and NC (5′-CUCAUUCCCAUAUGGUCGCAAG-3′) mimics were synthesized by Shanghai GenePharma Co., Ltd. Small interfering (si)RNA-TAFAZZIN (5′-GGAUACAGGAGAAAACGCA-3′) and si-TAFAZZIN-NC (siRNA NC) were obtained from Qiagen, Inc., to regulate the expression of TAFAZZIN. In accordance with the manufacturer's instructions, breast cancer cells were seeded into 96-well plates (4×104 cells/well). The cells were transfected using Lipofectamine® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) at room temperature for 10 min. The complexes were added dropwise on the cells and the plates were incubated at 37°C in 5% CO2. Following treatment of the breast cancer cells with miR-125a-5p for 6 days, the cells were then observed, and all images were captured on a Leica DM1 confocal microscope (magnification, ×200; Leica Microsystems, Inc.).
Mir 125a 5p mimics
Manufactured by GenePharma
Sourced in China
MiR-125a-5p mimics are synthetic RNA molecules designed to mimic the function of the natural miR-125a-5p microRNA. miR-125a-5p is a small non-coding RNA that plays a regulatory role in gene expression. The mimics are used in research applications to study the biological functions of miR-125a-5p.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mir 125a 5p mimics
1
Regulation of Breast Cancer by miR-125a-5p
2
Isolation and Culture of Mouse HSCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Primary mouse HSCs were isolated as reported previously [21] . HSCs were maintained in DMEM containing 10% fetal bovine serum (Gibco, Carlsbad, CA, USA), 100 U/mL penicillin and 100 μg/mL streptomycin (Gibco). Cells were cultured at 37°C in a humidified 5% CO 2 incubator. The miR-125a-5p inhibitor, miR-125a-5p mimics, miRNA negative control (miR-NC), siRNA against FIH1 (si-FIH1) and siRNA control (si-Ctrl) were obtained from GenePharma Corporation (Shanghai, China). Transfections were performed using Lipofectamine 2000 (Life Technologies, Carlsbad, CA, USA) according to the manufacturer's instructions.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!