Chip grade ab290

Manufactured by Abcam

Sourced in United Kingdom

ChIP grade ab290 is a laboratory equipment product manufactured by Abcam. It is designed for use in chromatin immunoprecipitation (ChIP) experiments. The product's core function is to facilitate the isolation and purification of protein-DNA complexes from cell or tissue samples.

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Lab products found in correlation

Morada, by Olympus (1 mentions) Odyssey imaging system, by LI COR (1 mentions) Irdye 800, by LI COR (1 mentions) Horseradish peroxidase, by Merck Group (1 mentions) Gene gun, by Bio-Rad (1 mentions) Ab128930, by Abcam (1 mentions) Ab15497, by Abcam (1 mentions) Ab198394, by Abcam (1 mentions) Cd437, by Merck Group (1 mentions) Antagomir, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Ab290 (560 citations) ChIP Grade (11 citations) Rabbit anti-GFP polyclonal antibody-Chip Grade (ab290) (2 citations)

4 protocols using chip grade ab290

Ultrastructural Localization of GFP

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Cells were fixed, embedded in gelatin, and cryosectioned as in Griffith et al. (2008) (link). Sections were then immuno-labeled using rabbit anti–GFP (chromatin immunoprecipitation [ChIP] grade ab290; Abcam), followed by protein A–gold detection. Sections were imaged in a FEI CM100bio electron microscope at 80 KV, equipped with a digital camera (Morada; Olympus).

Eisenberg-Bord M., Mari M., Weill U., Rosenfeld-Gur E., Moldavski O., Castro I.G., Soni K.G., Harpaz N., Levine T.P., Futerman A.H., Reggiori F., Bankaitis V.A., Schuldiner M, & Bohnert M. (2018). Identification of seipin-linked factors that act as determinants of a lipid droplet subpopulation. The Journal of Cell Biology, 217(1), 269-282.

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GFP-tagged Protein Extraction and Detection

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Cells expressing GFP tagged proteins and control cells were harvested by centrifugation, and proteins were extracted by NaOH or TCA extraction (Kushnirov, 2000 (link); Ast et al., 2013 (link)). Samples were analyzed by SDS-PAGE and Western blotting using an anti–GFP antibody (ChIP grade ab290; Abcam). Membranes were either probed with a secondary antibody coupled to horseradish peroxidase (0545; Sigma-Aldrich) for visualization by enhanced chemiluminescence, or with a secondary antibody conjugated to IRDye800 (LI-COR Biosciences), followed by scanning using the Odyssey Imaging System.

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Maize Transformation and ChIP-qPCR Analysis

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The construct pCAMBIA1302-P35S-ZmNF-YA1-GFP was used for the transformation of the young embryos of maize HiII via a gene gun (Bio-Rad, USA). Plants were produced from transgenic calli. Stable transgenic maize and the anti-GFP antibody (ChIP-grade ab290 from Abcam, Cambridge, UK) were used for the ChIP-qPCR assay. ChIP was performed with the method described originally by Kaufmann et al. (Kaufmann et al., 2010 (link)) with minor modifications and ChIP-qPCR were performed. The primers used to amplify the enriched region of the target genes are listed in Supplemental Table S8.

Yang Y., Wang B., Wang J., He C., Zhang D., Li P., Zhang J, & Li Z. (2022). Transcription factors ZmNF-YA1 and ZmNF-YB16 regulate plant growth and drought tolerance in maize. Plant Physiology, 190(2), 1506-1525.

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Characterization of Androgen Receptor Signaling

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DHT(Sigma), CD437 (Sigma), Enza (Sigma) mir-96 mimic and antagomir (Ambion, nocodazole (CST)
For Western immunoblots the following antibodies were used; Anti-RARγ (D3A4) rabbit mAb #8965 Cell Signaling Technology (CST); Anti-Myc Tag (9B11); Anti-TACC1 mouse mAb #2276 (CST); Anti-AR-V7 rabbit mAb #ab198394 (Abcam); Anti-AR (D6F11) rabbit mAb #5153 (CST); Anti-PGC-1α (3G6) Rabbit mAb #2178 (CST); Anti-REG4 Rabbit Polyclonal Antibody #43411 (CST); Anti-PMEPA1 rabbit polyclonal antibody # 16521–1-AP; Anti-ERRg rabbit mAb (EPR8100) #ab128930 (Abcam); Anti-DLC1 rabbit polyclonal antibody #PA5–53635 (Invitrogen); anti-PCNA rabbit polyclonal antibody #ab15497 (Abcam).
For rapid immunoprecipitation mass spectrometry of endogenous protein (RIME) assay RARγ (D3A4) #8965 CST antibody was used.
For CUT&RUN the following antibodies were used; Anti-Acetyl-Histone H3K27 (D5E4) XP^® rabbit mAb #8173 (CST); anti-GFP antibody, (ChIP Grade-ab290, Abcam); anti-AR (D6F11) XP^® rabbit mAb #5153 (CST), anti-IgG #2729S (CST), rabbit anti-H3S10ph #39254 (Active Motif).

Wani S.A., Hussain S., Gray J.S., Nayak D., Tang H., Perez L.M., Long M.D., Siddappa M., McCabe C.J., Sucheston-Campbell L.E., Freeman M.R, & Campbell M.J. (2024). Epigenetic disruption of the RARγ complex impairs its function to bookmark AR enhancer interactions required for enzalutamide sensitivity in prostate cancer. bioRxiv.

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