Monoclonal mouse anti phosphotyrosine py20

The following primary antibodies were used in this study: mouse monoclonal anti-CrkL (clone 5–6, Millipore, Billerica, MA, USA), mouse monoclonal anti-Crk (clone 22, BD Transduction Laboratories, San Jose, California, USA), rabbit monoclonal anti-phospho Akt (Ser473) (D9E, Cell Signaling Technology, Danver, MA, USA), mouse monoclonalanti-α-tubulin (DM1A, Sigma-Aldrich), rabbit polyclonal anti-Histone H3 (Cell Signaling Technology), monoclonal mouse anti-phosphotyrosine (PY20, Santa Cruz Biotechnology, Dallas, Texas, USA), and guinea-pig polyclonal anti-NS1 [3 (link)]. The secondary antibodies for Western blotting were: IRDye680CW goat anti-mouse IgG, IRDye680CW goat anti-rabbit IgG, and IRDye800CW goat anti-rabbit, and IRDye800CW rabbit anti-guinea pig were from LI-COR Biotechnology (Lincoln, NE, USA). Secondary antibodies for immunofluorescence staining were: AlexaFluor 488 goat anti-guinea pig IgG (Abcam, Cambridge, UK), and AlexaFluor 546 goat anti-mouse IgG (Molecular Probes, Eugene, OR, USA). Nuclei were stained with Hoechst.

The antibodies used are as follows: monoclonal mouse anti-actin (691001; MP Biomedicals, Santa Ana, CA); polyclonal rabbit anti-ErbB2 (HER2/neu), rabbit anti-nucleolin (C23), rabbit anti-Erk2 and rabbit anti-Akt, monoclonal mouse anti-phosphotyrosine (pY20) and monoclonal mouse anti-GFP and mouse anti-nucleolin (sc-284; sc-13057; sc-154; sc-8312; sc-508; sc-9996; sc-8031, respectively; Santa Cruz Biotechnology, Dallas, TX); monoclonal mouse anti-tubulin (T7816; Sigma-Aldrich); polyclonal rabbit anti-phospho-ErbB2, rabbit anti-phospho-Akt, rabbit anti-phospho-Erk1/2 (2249; 4058; 9101, respectively; Cell Signaling Technology, Danvers, MA); and monoclonal mouse anti-ErbB2 (extracellular; L87), which was a gift from Prof. Y. Yarden, Weizmann Institute of Science, Israel.
The aptamer GroA (AS1411) and the inactive oligomer Cro, were purchased from IDT (Jerusalem, Israel) as unmodified desalted oligonucleotides, as previously described [38 (link)].