Bacterially derived IL-6 was purchased from Calbiochem (San Diego, CA). Homo sapiens heme oxygenase-1 expression vector (HMOX1) was obtained from OriGene (Rockville, MD). Antibodies against both native STAT3 and STAT3 phosphorylated on tyrosine 705 or serine 725 were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). ECL reagents were purchased from Pierce (Rockford, IL). Luciferase assay kits were purchased from Promega (Madison, WI). Peroxidase-conjugated anti-rabbit and anti-mouse antibodies were obtained from Amersham (Arlington Heights, IL) and nitrocellulose was obtained from Schleicher & Schuell (Dassel, Germany). Methylene chloride (MC) was purchased from J.T. Baker (Phillipsburg, NJ). Tricarbonyl dichlororuthenium (II) dimer (TCDC) was purchased from Sigma (St. Louis, MO). All other reagents were purchased from Sigma (St. Louis, MO) and Merck (Darmstadt, Germany).
Tricarbonyl dichlororuthenium 2 dimer tcdc
Manufactured by Merck Group
Sourced in United States
Tricarbonyl dichlororuthenium (II) dimer (TCDC) is a laboratory reagent used in organic synthesis. It is a source of ruthenium, a transition metal. TCDC is commonly utilized as a catalyst in various chemical reactions.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using tricarbonyl dichlororuthenium 2 dimer tcdc
1
Cellular Signaling Pathway Regulation
2
Luciferase Assay for Nrf2 and NF-κB
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The p3xARE/Luc and NF-κB/Luc vectors were constructed by introducing the Nrf2 binding site or NF-κB binding site into the pGL3 promoter plasmid (Promega, Madison, WI, USA), respectively as described in a previous study (22) . Luciferase assay kits were purchased from Promega. Antibodies against HO-1 (SPA-896) and p65 (KAS-TF110) were purchased from Stressgen Biotechnologies (SB, San Diego, CA, USA). ICAM-1 (sc-7891), IκBα (sc-847), lamin B1 (sc-56143), α-tubulin (sc-53646) and Nrf2 (sc-722) antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Bacteriallyderived TNFα was purchased from Calbiochem (San Diego, CA, USA). All other reagents, including lycopene and tricarbonyl dichlororuthenium (II) dimer (TCDC) were purchased from Sigma (St. Louis, MO, USA). The CO donor, TCDC, was activated by adding the compound to culture medium to liberate CO (20) .
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