Horseradish peroxidase hrp conjugated anti mouse and anti rabbit secondary antibodies

Primary antibodies were rabbit anti-pCDK1^T14/15, mouse anti-CDK1, rabbit anti-pCDK2^T160, rabbit anti-CDK2, mouse anti-cMYC, mouse anti-survivin and rabbit anti- β-actin (Santacruz); anti-p21, anti-pRb, and anti-cleaved-PARP (Cell Signaling); mouse anti-cyclinB1 (BD Transduction). Secondary horseradish peroxidase (HRP)-conjugated anti-rabbit and anti-mouse antibodies were from Jackson ImmunoResearch Laboratories.

Dulbecco’s modified Eagle’s medium (DMEM), penicillin, and streptomycin were purchased from Cibco BRL (Paisley, Scotland, U.K.). Dimethyl sulphoxide (DMSO) and EDTA were purchased from Sigma Chemical (Poole, Dorset, U.K.). Potassium dichromate (K₂Cr₂O₇) and NAC were obtained from Merck Chemical Co. (Darmstadt, Germany). MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) was purchased from Sigma-Aldrich, Inc. (St. Louis, MO, U.S.A.). The antibodies for detecting anti-poly-(ADP-ribose) polymerase (PARP) antibody were obtained from Millipore (Billerica, MA, U.S.A.); anti-caspase-3 and anti-cleaved-caspase-3 antibodies were obtained from Epitomics (Burlingame, CA, U.S.A.); anti-LC3 antibody was obtained from Abgent (San Diego, CA, U.S.A.). Akt, phospho-Akt, p65, phospho-p65, IκB-α, ERK1/2, phospho-ERK1/2, p-38, phospho-p38, JNK and phospho-JNK were purchased from Cell Signaling (Beverly, MA, U.S.A.); GAPDH was obtained from Abcam Inc.(Cambridge, MA, U.S.A.); phospho- IkB-α was purchased from Upstate Biotechnology (Lake Placid, NY, U.S.A.). Horseradish peroxidase (HRP)-conjugated anti-mouse and anti-rabbit secondary antibodies were purchased from Jackson ImmunoResearch Laboratories Inc. (West Grove, PA, U.S.A.). TNF-α and IL-1α antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, U.S.A.).