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Fluorescein isothiocyanate isomer

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Fluorescein isothiocyanate isomer is a fluorescent dye used in various laboratory applications. It is a reactive derivative of fluorescein, a widely used fluorescent marker. The isomer provides a specific molecular structure that can be utilized for labeling proteins, antibodies, and other biomolecules for detection and analysis purposes.

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Lab products found in correlation

Dimethyl sulfoxide (dmso), by Merck Group (3 mentions) Tetraethyl orthosilicate, by Merck Group (2 mentions) Rhodamine b, by Merck Group (1 mentions) Pei 25k, by Merck Group (1 mentions) Pei 750k, by Merck Group (1 mentions) Bisbenzimide h 33342 trihydrochloride hoechst 33342, by Merck Group (1 mentions) Lipopolysaccharide lps, by Merck Group (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Sodium dodecyl sulfate (sds), by Maclin Biochemical Technology (1 mentions) Simvastatin, by Merck Group (1 mentions) N hydroxysuccinimide, by Merck Group (1 mentions) Resazurin, by Merck Group (1 mentions) Cetyltrimethylammonium bromide, by Maclin Biochemical Technology (1 mentions) 1 3 dimethylaminopropyl 3 ethylcarbodiimide hydrochloride edc, by Merck Group (1 mentions) Tetraethyl orthosilicate, by Maclin Biochemical Technology (1 mentions) 3 aminopropyl triethoxysilane, by Maclin Biochemical Technology (1 mentions) Guava easy cyte 8, by Merck Group (1 mentions) Propidium iodide, by Merck Group (1 mentions) Annexin 5, by Merck Group (1 mentions) 3 aminopropyltriethoxysilane, by Merck Group (1 mentions)

Spelling variants of this product

Fluorescein isothiocyanate isomer I (FITC) (45 citations) Fluorescein isothiocyanate isomer I (33 citations) Fluorescein isothiocyanate isomer (FITC, (3 citations) Fluorescein isothiocyanate (FITC) (isomer l) (2 citations)

6 protocols using fluorescein isothiocyanate isomer

1

Functionalized Nanomaterials Synthesis

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3-Mercaptopropyltrimethoxysilane (MPMS), 3-aminopropyltrimethoxysilane (APS), fluorescein isothiocyanate isomer (FITC), rhodamine B, bisbenzimide H 33342 trihydrochloride (Hoechst 33342) and branched polyethyleneimine solution [MW = 1.3k (PEI1.3k), 2.0k (PEI2.0k), 25k (PEI25k), and 750k (PEI750k)] were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Ammonium hydroxide (NH4OH, 28%) and ethanol were purchased from Kishida Chemical (Osaka, Japan). All other reagents and solvents were of analytical grade.
Nakamura M., Nakamura J., Mochizuki C., Kuroda C., Kato S., Haruta T., Kakefuda M., Sato S., Tamanoi F, & Sugino N. (2022). Analysis of cell–nanoparticle interactions and imaging of in vitro labeled cells showing barcorded endosomes using fluorescent thiol-organosilica nanoparticles surface-functionalized with polyethyleneimine. Nanoscale Advances, 4(12), 2682-2703.
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2

Multifunctional Nanocarriers for Targeted Drug Delivery

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All reagents were of analytical or HPLC grade and used without any further purification. Tetraethyl orthosilicate (TEOS), cetyl trimethyl ammonium bromide (CTAB), branched polyethyleneimine (PEI) (Mw = 10 kDa), (3-aminopropyl) triethoxysilane (APTES), and sodium dodecyl sulfate (SDS) were purchased from Macklin. Co., Ltd. Fluorescein isothiocyanate isomer (FITC), Simvastatin (SIM), hyaluronidase (HAase), N-hydroxy succinimide (NHS), 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), Resazurin, 4,6-diamidino-2-phenylindole (DAPI), and lipopolysaccharide (LPS) were purchased from Sigma-Aldrich Inc. (St. Louis, MO, USA). Sodium hyaluronate (HA) (Mw = 200~400 kDa) was purchased from Bloomage Biotechnology Co., Ltd. (Jinan, China). Oxidized low-density lipoprotein (ox-LDL) was purchased from Yiyuan Biotechnology Co., Ltd. (Bozhou, China). Phosphate buffer tablets were purchased from Amresco Inc. (Cleveland, OH, USA). The mouse ELISA kits to measure the secretion of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) levels were purchased from Beyotime Biotechnology Co., Ltd. (Shanghai, China).
Song K., Tang Z., Song Z., Meng S., Yang X., Guo H., Zhu Y, & Wang X. (2022). Hyaluronic Acid-Functionalized Mesoporous Silica Nanoparticles Loading Simvastatin for Targeted Therapy of Atherosclerosis. Pharmaceutics, 14(6), 1265.
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3

Annexin V Staining for Pyroptosis

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The cells in each group were collected, washed once with PBS, and then centrifuged. The supernatant was discarded, and the cells were washed one more time with PBS. Then the cells were stained with Annexin V, propidium iodide, and fluorescein isothiocyanate isomer (Sigma-Aldrich) in the dark for 30 minutes. Pyroptosis was assessed by flow cytometry (Guava easyCyte™ 8, Millipore, Burlington, VT, USA).
Zhao Y.J., Qiao H., Liu D.F., Li J., Li J.X., Chang S.E., Lu T., Li F.T., Wang D., Li H.P., He X.J, & Wang F. (2021). Lithium promotes recovery after spinal cord injury. Neural Regeneration Research, 17(6), 1324-1333.
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4

Synthesis and Characterization of Fluorescent Silica Nanoparticles

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Tetrachloroauric acid trihydrate (HAuCl4·3H2O, 99.9%), trisodium citrate dihydrate (99%), dodecyltrimethylammonium bromide (DTAB, 98%), polyvinylpyrrolidone (PVP, Mw ~ 55,000), ethylene glycol (EG, 99.8%), ammonium hydroxide (28–30% NH3 basis), tetraethyl orthosilicate (TEOS, 99%), dimethyl sulfoxide (DMSO, 99.9%), fluorescein isothiocyanate isomer (FITC, 90%), and (3-aminopropyl) triethoxysilane (APTES, 99%) were purchased from Sigma Aldrich. Fetal Bovine Serum (FBS), Trypsin–EDTA (0.25% solution), a penicillin streptomycin solution, RPMI 1640 media, and phosphate buffered saline (PBS) were purchased from Gibco. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was purchased from Thermofisher. Deionized water (18.2 MΩ/cm) and ethanol (EtOH, 99%) were used as solvents. All reagents were used without further purification.
Kim J., Chun S.H., Amornkitbamrung L., Song C., Yuk J.S., Ahn S.Y., Kim B.W., Lim Y.T., Oh B.K, & Um S.H. (2020). Gold nanoparticle clusters for the investigation of therapeutic efficiency against prostate cancer under near-infrared irradiation. Nano Convergence, 7, 5.
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5

Protein Modification and Characterization

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Hydrochloric acid (37%), sodium
dodecyl sulfate (98.5%), glycerol (>99%), 1,4-dithiothreitol (>98%),
bromophenol blue, tetraethyl orthosilicate (98%), fluorescein isothiocyanate
isomer (>90%), 3-aminopropyltrimethoxysilane (>97%), ammonia
(35%),
ethanol (99.8%), acetic acid (analytical standard), 2-mercaptoethanol
(>99%), (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES,
99.5%), glycine (>99%), succinic anhydride (>99%), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide
hydrochloride (EDC), N-hydroxysulfosuccinimide sodium
salt (>98%), NaCl (>99.5%), Tween 20, and dimethylsulfoxide
(>99.9%)
were purchased from Sigma-Aldrich and used without further purification.
Multicolor broad range protein ladder, Micro BCA assay kit and Pierce
LAL chromogenic endotoxin quantitation kit were purchased from Thermo
Fisher Scientific. NHS-PEG8-Maleimide was purchased from Irish Biotech
GMBH. Normal Human serum was purchased from Merck-Millipore Inc.
Behan J.A., Xie Z., Wang Y.F., Yang X., Aastrup T., Yan Y., Adumeau L, & Dawson K.A. (2023). Quartz Crystal Microbalance Method to Measure Nanoparticle–Receptor Interactions and Evaluate Nanoparticle Design Efficiency. JACS Au, 3(6), 1623-1633.
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6

Fabrication of miRNA-Loaded Hyaluronic Acid Hydrogel

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Sodium hyaluronate (Mw 15 kDa) was purchased from Lifecore Biomedical Inc., Chaska, MN. Polyvinylpyrrolidone (PVP, Mw 1300000), Dowex H+ 50W × 8–100 sulfonic resin, N-Hydroxysuccinimide (NHS, 98%), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDAC, ≥99%), 2-(N-Morpholino)ethanesulfonic acid (MES, ≥99%), cystamine dihydrochloride (CADH, >98%), α-bromoisobutyric acid (BIBA, 98%), dimethyl sulfoxide (DMSO, ≥99.5%), trimethylamine (TEA, ≥99%), copper(I) bromide (CuBr, 99%), glycidyl methacrylate (GMA, 98%), ethanolamine (EA, 98%), polyethylenimine (PEI, 25 kDa), and fluorescein isothiocyanate isomer (FITC, 97.5%) were obtained from Sigma-Aldrich Chemical Co., St. Louis, MO. N,N,N’,N’,N”-pentamethyldiethylenetriamine (PMDETA, ≥99%) and ethylenediaminetetraacetic acid (EDTA, 99%) were obtained from Acros Organics N.V., New Jersey, US. GMA was used after removal of the inhibitors in a ready-to-use disposable inhibitor-removal column. Poly(lactic-co-glycolic acid) (PLGA, 50:50 lactide:glycolide) was purchased from Lactel, Birmingham, AL. Rno-miR-26a-5p (miRNA mimic) was purchased from ThermoFisher Scientific International Inc, Massachusetts, US.
Li R., Wang H., John J.V., Song H., Teusink M.J, & Xie J. (2020). 3D Hybrid Nanofiber Aerogels Combining with Nanoparticles Made of a Biocleavable and Targeting Polycation and MiR-26a for Bone Repair. Advanced functional materials, 30(49), 2005531.
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