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Mtor ab32028

Manufactured by Abcam
Sourced in United States

The mTOR (ab32028) is a laboratory product manufactured by Abcam. It is an antibody that can be used to detect the mammalian target of rapamycin (mTOR) protein. mTOR is a serine/threonine protein kinase that regulates cell growth, cell proliferation, cell motility, cell survival, protein synthesis, and transcription.

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2 protocols using mtor ab32028

1

Protein Expression Analysis Protocol

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GAPDH (6c5), E-cadherin (H-108), Vimentin (V9) and MMP-2 (H-76) antibodies were purchased from Santa Cruz Biotechnology (PasoRobles, CA). Phospho-Akt (Ser473 D9E) and Akt (11E7) antibodies were purchased from Cell Signaling Technology Company (Boston, MA), Phospho-mTOR (ab109268) and mTOR (ab32028) antibodies were from Abcam Company (San Diego, CA). CD68 (M087601-2) antibody were purchased from DAKO (Carpinteria, CA). Rapamycin was from Cell Signaling Technology Company (Boston, MA). Crystal violet was from Fishers Scientific Company (Grand Island, NY). Anti mouse/rabbit second antibody for Western Blot and Lipofectamine 2000 transfection reagent were purchased from Life Technologies Company (Grand Island, NY).
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2

Antibody Detection in Cell Signaling

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The antibody LC3B (#3868) was purchased from Cell Signaling Technology, Inc (USA). The antibodies p62 (ab109012), Beclin-1 (ab210498), mTOR (ab32028), and p-mTOR (S2448) (ab109268) were purchased from Abcam (USA). The antibody α-tubulin (AF0001 and AT819) and secondary antibodies, including horse radish peroxidase (HRP)-labeled goat anti-mouse IgG (H+L) (A0216), HRP-labeled goat anti-rabbit IgG (H+L) (A0208), Alexa Fluor 647-labeled goat anti-rabbit IgG (H+L) (A0468), and Alexa Fluor 488-labeled goat anti-mouse IgG (H+L) (A0428), were purchased from Beyotime (China).
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