High-performance liquid chromatographic separation and mass spectrometric detection were performed using Waters Quattro Premier XE/Acquity UPLC system coupled to a tandem quadrupole mass spectrometer (Waters Corporation, Milford, MA, United States). Lab solutions LCMS software (Masslynx V4.1) was used to control the instruments and process the data. Please see details as we previously described [19 (link)].
Quattro premier xe acquity uplc system
Manufactured by Waters Corporation
Sourced in United States
The Quattro Premier XE/Acquity UPLC system is a high-performance liquid chromatography (HPLC) instrument designed for sensitive and accurate analysis of complex samples. It combines the Quattro Premier XE mass spectrometer and the Acquity UPLC system to provide a comprehensive solution for analytical workflows.
Automatically generated - may contain errors
4 protocols using quattro premier xe acquity uplc system
1
Liquid Chromatography-Mass Spectrometry Protocol
2
Liquid Chromatography-Mass Spectrometry Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Liquid chromatographic separation and mass spectrometric detection were performed using Waters Quattro Premier XE/Acquity UPLC system coupled to a tandem quadrupole mass spectrometer (Waters Corporation, Milford, MA, United States). Lab solutions LCMS software (Masslynx V4.1) was used to control the instruments and process the data. This instrument was equipped with both ESI and APCI sources. Please see more details as we previously described (Pan et al., 2019 (link)).
3
Plasma Drug Quantification by UPLC-MS/MS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Plasma drug concentrations were measured using liquid chromatography coupled with tandem mass spectrometry (ACQUITY UPLC-Quattro Premier XE system, Waters, Milford, MA, USA) in a device equipped with an ACQUITY UPLC BEH shield (RP18, 1.7 μm) and a 2.1×100 mm column (Waters). All the data were acquired and processed using MassLynx software (version 4.1) with QuanLynx (Waters). Positive-ion electrospray tandem mass spectroscopy, operated under the multiple reaction monitoring mode, was used to detect mass transitions (parent to daughter ion), with m/z 399.48 to 283.1 for sunitinib, m/z 371.27 to 283.1 for N-desethylsunitinib, m/z 465.35 to 252.1 for sorafenib, and m/z 268.23 to 116.03 for metoprolol, which served as an internal standard. Plasma samples were deproteinized with 4 volumes of acetonitrile (Nacalai Tesque) and the supernatants were used for analysis.
4
UPLC-MS Identification of Resorcinol Polyphenols
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In the research, UPLC-MS was applied to the identification of RP. The analysis was operated on an Acquity UPLC-Quattro Premier XE system (Waters, Massachusetts, USA). The sample separation was performed applying a UPLC HSS C18 analytical column (2.1 mm × 100 mm, 1.7 µm) (Waters, Massachusetts, USA) at 45 °C, the flow rate was 0.25 mL/min, and the injection volume was 2 µL. The mobile phase included methanol (solvent A) and 0.1% (v/v) formic acid in water (solvent B). The elution gradient started with 10–35% A to 15 min, 35–45% A to 40 min (end analysis). Mass spectra parameters were set as follows: negative and positive ion mode for a mass range of m/z 180 to m/z 800, capillary voltage 2.8 kV, cone voltage 20 V, source temperature 100 °C, desolvation temperature 250 °C, cone gas flow 40 L/h, desolvation gas flow 500 L/h.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!