Ld plan neofluar 20 0.4 korr ph2 objective

Manufactured by Zeiss

The LD Plan-Neofluar 20×/0.4 Korr Ph2 objective is a high-performance microscope objective from Zeiss. It has a magnification of 20x and a numerical aperture of 0.4. The objective is designed for use with phase contrast microscopy techniques.

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Lab products found in correlation

Axio observer z1, by Zeiss (2 mentions) Axiocam mrm camera, by Zeiss (2 mentions) Axiovision, by Zeiss (2 mentions)

Spelling variants of this product

Plan-Neofluar (124 citations) Plan-Neofluar objective (53 citations) LD Plan-Neofluar (12 citations) LD Plan-Neofluar 20×/0.4 objective (5 citations) Plan Neofluar 20× objective (4 citations) LD Plan-Neofluar 20× objective (3 citations) Plan-NEOFLUAR 20×/0.4 objective (2 citations) Neofluar objectives (2 citations) LD Plan-Neofluar 20×/0.4 Korr Ph2 objective lens (2 citations)

2 protocols using ld plan neofluar 20 0.4 korr ph2 objective

Mammary Organoid Time-lapse Imaging

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Time-lapse imaging of mammary organoids was conducted using an LD Plan-Neofluar 20×/0.4 Korr Ph2 objective lens and a Cell Observer system with an AxioObserver Z1 and an AxioCam MRM camera (Carl Zeiss). In general, we recorded 100–200 positions in parallel for 5–7 d, with images acquired at 20-min intervals. Temperature was maintained at 37°C and CO₂ at 5%. AxioVision (Carl Zeiss) was used to acquire and analyze time-lapse movies, place scale bars, and export individual TIFFs. Photoshop CS5 (Adobe) was used to adjust levels on entire images to maximize image clarity.

Shamir E.R., Pappalardo E., Jorgens D.M., Coutinho K., Tsai W.T., Aziz K., Auer M., Tran P.T., Bader J.S, & Ewald A.J. (2014). Twist1-induced dissemination preserves epithelial identity and requires E-cadherin. The Journal of Cell Biology, 204(5), 839-856.

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Time-lapse Imaging of Mammary Organoids

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Time-lapse imaging of mammary organoids was conducted using an LD Plan-Neofluar 20×/0.4 Korr Ph2 objective lens and a Cell Observer system with an AxioObserver Z1 and an AxioCam MRM camera (Carl Zeiss). Images were acquired at 20-min intervals for 5-7 days. Temperature was maintained at 37°C and CO₂ at 5%. AxioVision (Carl Zeiss) was used to analyze both fixed images and time-lapse movies, place scale bars, and export individual TIFFs. Adobe Photoshop was used to adjust levels on entire images to maximize image clarity.

Shamir E.R., Coutinho K., Georgess D., Auer M, & Ewald A.J. (2016). Twist1-positive epithelial cells retain adhesive and proliferative capacity throughout dissemination. Biology Open, 5(9), 1216-1228.

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