F12 ham medium

NOB (>95.0% purity) and cobalt chloride hexahydrate were purchased from Aladdin Shanghai Biochemical Technology (Shanghai, China). Recombinant human p53 protein was obtained from Boston Biochem (Beijing, China). F12 (Ham) medium, Dulbecco’s modification of Eagle’s medium Dulbecco (DMEM) (high glucose), and dimethyl sulfoxide (DMSO) were obtained from Boster Biological Technology (Pleasanton, CA, USA). Fetal bovine serum (FBS) and Dulbecco’s phosphate-buffered saline were obtained from Corning (New York, NY, USA). Trypsin-Ethylene diamine tetraacetic acid (EDTA) solution, trypsin without EDTA, penicillin, and streptomycin were purchased from Keygen (Beijing, China). JEG-3 and BeWo cells were purchased from Peking Union Medical College (Beijing, China).

NOB (≥95% by HPLC) was obtained from Aladdin Biochemical Technology (Shanghai, China). F12 (Ham) medium and dimethyl sulfoxide (DMSO) were obtained from Boster Biological Technology (Pleasanton, CA, USA). Fetal bovine serum (FBS) was provided by Corning (New York, NY, USA). The CCK-8 kit was purchased from Multi Sciences (Hangzhou, China). A mixture of penicillin and streptomycin, trypsin, Annexin V-FITC/propidium iodide (PI) apoptosis detection kit, cell cycle detection kit, and BCA protein assay kit were obtained from Keygen (Beijing, China). Cold radio immune precipitation assay (RIPA) buffer and phenylmethylsulfonyl fluoride (PMSF) were obtained from Beyotime (Shanghai, China). The antibodies used for the Western blot assay were all purchased from Abcam (Cambridge, UK). The ECL reagent kit was from GE Healthcare (Buckinghamshire, UK). Other chemicals were of analytical grade and used without further purification.