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Cd19 apc cy7

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The CD19 APC-Cy7 is a fluorescently conjugated antibody that binds to the CD19 cell surface antigen. It is commonly used in flow cytometry applications to identify and analyze B cells.

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Lab products found in correlation

Ebio1d3, by Thermo Fisher Scientific (2 mentions) Cd3ε pecy7, by Thermo Fisher Scientific (2 mentions) Cyan adp, by Agilent Technologies (2 mentions) Cd21 pe cy5, by BD (2 mentions) Cd138 apc, by Miltenyi Biotec (2 mentions) Cd3 pacific orange, by Thermo Fisher Scientific (2 mentions) Goat f ab 2 anti human igd fitc, by Thermo Fisher Scientific (2 mentions) Goat f ab 2 anti human igm pe cy, by Thermo Fisher Scientific (2 mentions) Cd14 pacific orange, by Thermo Fisher Scientific (2 mentions) Cd24 pe a610, by Thermo Fisher Scientific (2 mentions) Cd38 pacific blue, by BD (2 mentions) Cd23 pe cy7, by BD (2 mentions) Cd27 pe, by BD (2 mentions) Facsaria 2, by BD (2 mentions) Stereomicroscope, by Leica (1 mentions) Facs verse flow cytometer, by Tree Star (1 mentions) Ly6g pecy7, by Thermo Fisher Scientific (1 mentions) Cd11b pe cy7 m1 70, by Thermo Fisher Scientific (1 mentions) Dnase 1, by Roche (1 mentions) Collagenase d, by Roche (1 mentions)

5 protocols using cd19 apc cy7

1

Flow Cytometry Cell Immunophenotyping

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Single cells suspensions were stained for 30 minutes in PBS (with 0.5% BSA and 2 mM EDTA) with ‘cocktails’ of the following antibodies CD3ε PECy7 (1:200) (145-2C11), CD19 APC-Cy7 (1:100) (eBio1D3) (from EBiosciences). Cells were then washed twice in PBS (with 0.5% BSA and 2 mM EDTA), resuspended in PBS (with 0.5% BSA and 2 mM EDTA) and then analyzed using a Cyan-ADP (Dako) with forward/side scatter gates set to exclude non-viable cells. Data were analyzed with FlowJo software (Tree Star, Oregon, USA).
Chimen M., McGettrick H.M., Apta B., Kuravi S.J., Yates C.M., Kennedy A., Odedra A., Alassiri M., Harrison M., Martin A., Barone F., Nayar S., Hitchcock J.R., Cunningham A.F., Raza K., Filer A., Copland D.A., Dick A.D., Robinson J., Kalia N., Walker L.S., Buckley C.D., Nash G.B., Narendran P, & Rainger G.E. (2015). Homeostatic regulation of T cell trafficking by a B cell derived peptide is impaired in autoimmune and chronic inflammatory disease. Nature medicine, 21(5), 467-475.
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2

Flow Cytometry Cell Immunophenotyping

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Single cells suspensions were stained for 30 minutes in PBS (with 0.5% BSA and 2 mM EDTA) with ‘cocktails’ of the following antibodies CD3ε PECy7 (1:200) (145-2C11), CD19 APC-Cy7 (1:100) (eBio1D3) (from EBiosciences). Cells were then washed twice in PBS (with 0.5% BSA and 2 mM EDTA), resuspended in PBS (with 0.5% BSA and 2 mM EDTA) and then analyzed using a Cyan-ADP (Dako) with forward/side scatter gates set to exclude non-viable cells. Data were analyzed with FlowJo software (Tree Star, Oregon, USA).
Chimen M., McGettrick H.M., Apta B., Kuravi S.J., Yates C.M., Kennedy A., Odedra A., Alassiri M., Harrison M., Martin A., Barone F., Nayar S., Hitchcock J.R., Cunningham A.F., Raza K., Filer A., Copland D.A., Dick A.D., Robinson J., Kalia N., Walker L.S., Buckley C.D., Nash G.B., Narendran P, & Rainger G.E. (2015). Homeostatic regulation of T cell trafficking by a B cell derived peptide is impaired in autoimmune and chronic inflammatory disease. Nature medicine, 21(5), 467-475.
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3

Flow Cytometry Analysis of Mouse Brain Immune Cells

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After perfusion with saline, the ChP was removed under a stereomicroscope (Leica) and collected in 200 µl Dulbecco's Modified Eagle Medium (DMEM) + 10% fetal calf serum (FCS). ChP cells were isolated by incubating the samples in 2 ml of digestion mix [DMEM + 10% FCS + 0.4% DNASEI (#11284932001, Roche) + 3% CollagenaseD (#11088866001, Roche)], 10 min at 37 °C, and then mechanically dissociated. Brain homogenates of both hemispheres were prepared by the same dissociation/digestion protocol. Cerebral mononuclear cells were subsequently isolated using a 70 and 40% discontinuous Ficoll gradient. The following mouse antigen-specific antibodies were purchased from eBioscience: CD45 eF450 (30-F11), CD11b PE-Cy7 (M1/70), CD3 V510 or FITC (17A2), CD19 APC-Cy7 (eBisID3), CD8 PE (53-6.7), CD4 PerCP5.5 (45-0042-82), Ly6C APC (HK1.4), Ly6G PE Cy7 (RB6-8C5) and Ly6G PE (1A8). To quantify the various cell populations, cells were stained with specific antibodies in accordance with the manufacturer's protocols, acquired in a FACSVerse flow cytometer and analyzed with FlowJo software (version 10, Tree Star).
Llovera G., Benakis C., Enzmann G., Cai R., Arzberger T., Ghasemigharagoz A., Mao X., Malik R., Lazarevic I., Liebscher S., Ertürk A., Meissner L., Vivien D., Haffner C., Plesnila N., Montaner J., Engelhardt B, & Liesz A. (2017). The choroid plexus is a key cerebral invasion route for T cells after stroke. Acta neuropathologica, 134(6).
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4

Flow Cytometric Analysis of Human B Cell Subsets

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Mononuclear cells were isolated from peripheral blood using ficoll density gradient centrifugation and stained with the following anti-human antibody staining reagents: Goat F(ab')2 Anti-Human IgD- FITC (Invitrogen) or -APC (clone: IgD26, Miltenyi Biotec); Goat F(ab')2 Anti-Human IgM-PE-Cy5; CD3-Pacific Orange (clone: UCHT1), CD14-Pacific Orange (clone: TK4); CD24-PE-A610 (clone: SN3; Invitrogen); CD19-APC-Cy7 (clone: SJ25C1); CD38-Pacific Blue (clone: HB7); CD23-PE-Cy7 clone: M-L233); CD21-PE-Cy5 (clone B-ly4); CD27-PE (clone: L128, BD Pharmingen); CD138-APC (clone: 44F9, Miltenyi Biotec) and mitotracker green. Approximately 0.1–3 × 105 cells were collected for each population using a BD FACS Aria II (BD Biosciences).
Tipton C.M., Fucile C.F., Darce J., Chida A., Ichikawa T., Gregoretti I., Schieferl S., Hom J., Jenks S., Feldman R.J., Mehr R., Wei C., Lee F.E., Cheung W.C., Rosenberg A.F, & Sanz I. (2015). Diversity, cellular origin and autoreactivity of antibody-secreting cell expansions in acute Systemic Lupus Erythematosus. Nature immunology, 16(7), 755-765.
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5

Flow Cytometric Analysis of Human B Cell Subsets

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Mononuclear cells were isolated from peripheral blood using ficoll density gradient centrifugation and stained with the following anti-human antibody staining reagents: Goat F(ab')2 Anti-Human IgD- FITC (Invitrogen) or -APC (clone: IgD26, Miltenyi Biotec); Goat F(ab')2 Anti-Human IgM-PE-Cy5; CD3-Pacific Orange (clone: UCHT1), CD14-Pacific Orange (clone: TK4); CD24-PE-A610 (clone: SN3; Invitrogen); CD19-APC-Cy7 (clone: SJ25C1); CD38-Pacific Blue (clone: HB7); CD23-PE-Cy7 clone: M-L233); CD21-PE-Cy5 (clone B-ly4); CD27-PE (clone: L128, BD Pharmingen); CD138-APC (clone: 44F9, Miltenyi Biotec) and mitotracker green. Approximately 0.1–3 × 105 cells were collected for each population using a BD FACS Aria II (BD Biosciences).
Tipton C.M., Fucile C.F., Darce J., Chida A., Ichikawa T., Gregoretti I., Schieferl S., Hom J., Jenks S., Feldman R.J., Mehr R., Wei C., Lee F.E., Cheung W.C., Rosenberg A.F, & Sanz I. (2015). Diversity, cellular origin and autoreactivity of antibody-secreting cell expansions in acute Systemic Lupus Erythematosus. Nature immunology, 16(7), 755-765.
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