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Prp19

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Prp19 is a protein complex that plays a key role in the spliceosome, which is responsible for the removal of introns from pre-mRNA. Prp19 is involved in the activation of the spliceosome and the release of the mature mRNA for translation.

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Lab products found in correlation

Vinculin, by Merck Group (2 mentions) U2af1, by Fortis Life Sciences (2 mentions) Snrpf, by Abcam (2 mentions) Bud31, by Proteintech (2 mentions) Sf3a1, by Fortis Life Sciences (2 mentions) Eftud2, by Fortis Life Sciences (2 mentions) Eif2s1, by Abcepta (2 mentions) Eif3i p36, by BioLegend (2 mentions) Sf3b1, by Fortis Life Sciences (2 mentions) C myc, by Cell Signaling Technology (2 mentions) Flag tag (flag), by Merck Group (2 mentions) Cleaved caspase 3, by Cell Signaling Technology (2 mentions)

2 protocols using prp19

1

Comprehensive Protein Analysis by Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed in 1× SDS sample buffer (62.5mM Tris-HCl, pH6.8, 10% glycerol, 2% SDS, 2.5% β-mercaptoethanol) and heated at 95°C for 12 minutes. The following antibodies were used for Western blotting: Flag (Sigma, A8592), BUD31 (ProteinTech, 11798-1-AP), SF3B1 (Bethyl, A300-996A), Prp19 (Bethyl, A300-101A), U2AF1 (Bethyl, A302-079A), SF3A1 (Bethyl, A301-603A), EFTUD2 (Bethyl, A300-957A), SNRPF (Abcam, 154870), HER2 (Millipore, 06-562), EGFR (Cell Signaling, 2232), cleaved caspase-3 (Cell Signaling, 9664), RPS8 (Assay Biotechnology, R12-3466), EIF2S1 (Abgent, AP13469s), eIF3I (p36) (Biolegend, 646701), and c-Myc (D84C12) (Cell Signaling, 5605). Vinculin (Sigma, V9131) and ran (BD Biosciences, 610340) was used as loading control.
Hsu T.Y., Simon L.M., Neill N., Marcotte R., Sayad A., Bland C.S., Echeverria G.V., Sun T., Kurley S.J., Tyagi S., Karlin K.L., Dominguez-Vidaña R., Hartman J.D., Renwick A., Scorsone K., Bernardi R.J., Skinner S.O., Jain A., Orellana M., Lagisetti C., Golding I., Jung S.Y., Neilson J.R., Zhang X.H., Cooper T.A., Webb T.R., Neel B.G., Shaw C.A, & Westbrook T.F. (2015). The spliceosome is a therapeutic vulnerability in MYC-driven cancer. Nature, 525(7569), 384-388.
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2

Comprehensive Protein Analysis by Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed in 1× SDS sample buffer (62.5mM Tris-HCl, pH6.8, 10% glycerol, 2% SDS, 2.5% β-mercaptoethanol) and heated at 95°C for 12 minutes. The following antibodies were used for Western blotting: Flag (Sigma, A8592), BUD31 (ProteinTech, 11798-1-AP), SF3B1 (Bethyl, A300-996A), Prp19 (Bethyl, A300-101A), U2AF1 (Bethyl, A302-079A), SF3A1 (Bethyl, A301-603A), EFTUD2 (Bethyl, A300-957A), SNRPF (Abcam, 154870), HER2 (Millipore, 06-562), EGFR (Cell Signaling, 2232), cleaved caspase-3 (Cell Signaling, 9664), RPS8 (Assay Biotechnology, R12-3466), EIF2S1 (Abgent, AP13469s), eIF3I (p36) (Biolegend, 646701), and c-Myc (D84C12) (Cell Signaling, 5605). Vinculin (Sigma, V9131) and ran (BD Biosciences, 610340) was used as loading control.
Hsu T.Y., Simon L.M., Neill N., Marcotte R., Sayad A., Bland C.S., Echeverria G.V., Sun T., Kurley S.J., Tyagi S., Karlin K.L., Dominguez-Vidaña R., Hartman J.D., Renwick A., Scorsone K., Bernardi R.J., Skinner S.O., Jain A., Orellana M., Lagisetti C., Golding I., Jung S.Y., Neilson J.R., Zhang X.H., Cooper T.A., Webb T.R., Neel B.G., Shaw C.A, & Westbrook T.F. (2015). The spliceosome is a therapeutic vulnerability in MYC-driven cancer. Nature, 525(7569), 384-388.
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