DMSO (analytical reagent grade), poly(methyl vinyl ether-alt-maleic anhydride) (average Mn ≈ 80,000 Da, impurities: <2% benzene), Dulbecco’s phosphate-buffered saline (1.15 g L−1 dibasic sodium phosphate, 0.2 g L−1 potassium chloride, 8 g L−1 sodium chloride, and 0.2 g L−1 monobasic potassium phosphate) (DPBS) were purchased from Sigma-Aldrich. Trehalose dihydrate (purity >98%) was purchased from Carbosynth. Poly-(ethylene glycol) (PEG) (average Mn = 4000 g mol−1) was purchased from Sigma-Aldrich. 2-Dimethylaminoethanol (purity 99%) was purchased from Acros Organics. Tetrahydrofuran (laboratory reagent grade) was purchased from Merck. BD FACSFlow sheath fluid was purchased from BD biosciences. Sheep’s blood in Alsever’s solution (not defibrinated) was from TCS Biosciences. Polyampholyte (poly(vinyl ether-alt-maleic acid mono(dimethylamino ethyl)ester)) was synthesized in house as described in the Supporting Information Milli-Q ultrapure water (>18.2 MΩ cm−1 2 ppb) was used throughout.
Poly methyl vinyl ether alt maleic anhydride
Manufactured by Merck Group
Sourced in United States
Poly(methyl vinyl ether-alt-maleic anhydride) is a copolymer composed of methyl vinyl ether and maleic anhydride monomers. It is a white, odorless powder with a high molecular weight. The material exhibits good thermal stability and film-forming properties.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco s phosphate buffered saline, by Merck Group (3 mentions)
Tetrahydrofuran, by Merck Group (2 mentions)
Trehalose dihydrate, by Biosynth (2 mentions)
2 dimethylaminoethanol, by Thermo Fisher Scientific (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Dimethylaminoethanol, by Merck Group (2 mentions)
Facsflow sheath fluid, by BD (1 mentions)
3 aminopropyltriethoxysilane, by Merck Group (1 mentions)
Maleic anhydride, by Merck Group (1 mentions)
Tetraethyl orthosilicate, by Merck Group (1 mentions)
3 triethoxysilyl propyl isocyanate, by Merck Group (1 mentions)
Deferoxamine mesylate salt, by Merck Group (1 mentions)
Sodium chloride (nacl), by Thermo Fisher Scientific (1 mentions)
Preformulated tablets, by Merck Group (1 mentions)
Polyethylene glycol peg, by Merck Group (1 mentions)
Ham s f 12k media, by Thermo Fisher Scientific (1 mentions)
Cellevent caspase 3 7 green detection reagent, by Thermo Fisher Scientific (1 mentions)
Advanced dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Poly ethylene glycol methyl ether, by Merck Group (1 mentions)
6 protocols using poly methyl vinyl ether alt maleic anhydride
1
Synthesis and Characterization of Polyampholyte Nanoparticles
2
Silica-based Bioconjugation Reagents
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The majority of the reagents
were obtained from Sigma Aldrich (Saint Louis, USA) (deferoxamine
mesylate salt ≥92.5%; poly(methyl vinyl ether-alt-maleic anhydride) of average Mw ∼216,000
Da and Mn ∼80,000 Da; tetraethyl
orthosilicate ≥99.0%, 3-(triethoxysilyl)propyl isocyanate 95%;
(3-aminopropyl)triethoxysilane 97%; maleic anhydride 99%; 1,1,1,3,3,3-hexamethyldisilazane
for synthesis ≥98%; ZnCl2 ≥98%, anhydrous;
Fe(ClO4)3·xH2O, low chloride; protoporphyrin IX (PPIX) ≥95%; human serum
from human male AB plasma, USA origin, sterile-filtered). Silica modified
with surface isocyanate and maleimide groups was obtained from SiliCycle
Inc. (Quebec, Canada) and characterized as follows: SiO2–maleimide (size: 40–63 μm, loading: 0.68 mmol
g–1) and SiO2–isocyanate (size:
40–63 μm, loading: 1.41 mmol g–1).
The other substances were of purity grade p.a. and obtained from POCH
(Gliwice, Poland) (FeCl3·6H2O ≥97%;
Na2HPO4·2H2O ≥99%; NaH2PO4·H2O ≥99%; Et2O 99.5%; KCl 99.5%; anhydrous EtOH 99.8%; NH4OH 25%),
Stanlab (Lublin, Poland) (HCl 35–38%; citric acid monohydrate;
toluene), and EUROCHEM (Tarnów, Poland) (NaCl 99.5%; DMF, DCM).
Moreover, (NH4)2Fe(SO4)2·6H2O was supplied by Aktyn (Suchy Las, Poland),
and DMSO was purchased from Merck (Darmstadt, Germany), which was
dried over molecular sieves 4 Å prior to its use.
were obtained from Sigma Aldrich (Saint Louis, USA) (deferoxamine
mesylate salt ≥92.5%; poly(methyl vinyl ether-alt-maleic anhydride) of average Mw ∼216,000
Da and Mn ∼80,000 Da; tetraethyl
orthosilicate ≥99.0%, 3-(triethoxysilyl)propyl isocyanate 95%;
(3-aminopropyl)triethoxysilane 97%; maleic anhydride 99%; 1,1,1,3,3,3-hexamethyldisilazane
for synthesis ≥98%; ZnCl2 ≥98%, anhydrous;
Fe(ClO4)3·xH2O, low chloride; protoporphyrin IX (PPIX) ≥95%; human serum
from human male AB plasma, USA origin, sterile-filtered). Silica modified
with surface isocyanate and maleimide groups was obtained from SiliCycle
Inc. (Quebec, Canada) and characterized as follows: SiO2–maleimide (size: 40–63 μm, loading: 0.68 mmol
g–1) and SiO2–isocyanate (size:
40–63 μm, loading: 1.41 mmol g–1).
The other substances were of purity grade p.a. and obtained from POCH
(Gliwice, Poland) (FeCl3·6H2O ≥97%;
Na2HPO4·2H2O ≥99%; NaH2PO4·H2O ≥99%; Et2O 99.5%; KCl 99.5%; anhydrous EtOH 99.8%; NH4OH 25%),
Stanlab (Lublin, Poland) (HCl 35–38%; citric acid monohydrate;
toluene), and EUROCHEM (Tarnów, Poland) (NaCl 99.5%; DMF, DCM).
Moreover, (NH4)2Fe(SO4)2·6H2O was supplied by Aktyn (Suchy Las, Poland),
and DMSO was purchased from Merck (Darmstadt, Germany), which was
dried over molecular sieves 4 Å prior to its use.
3
Phosphate-Buffered Saline Preparation and Polymer Characterization
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Phosphate-buffered saline
(PBS) solutions were prepared using pre-formulated tablets (Sigma-Aldrich)
in 200 mL of Milli-Q water (>18.2 Ω mean resistivity) to
give [NaCl] = 0.138 M, [KCl] = 0.0027 M, and pH 7.4. Poly(methyl vinyl
ether-alt-maleic anhydride) Mn 80 000 and 311 000 g.mol–1, dimethylaminoethanol, and N-[4-(aminomethyl)benzyl]rhodamine
6G-amide bis(trifluoroacetate) were purchased from Sigma-Aldrich.
Poly(methyl vinyl ether-alt-maleic anhydride) average Mn 20 000 g mol–1 was
purchased from Scientific Polymer Products; the dispersity of polymerP2 from the manufacturer is 2.7 and that of P3 is 3.47; the supplier of P1 did not list the Mw value, only the Mn. Sodium chloride was purchased from Fisher Scientific. All solvents
were purchased from VWR or Sigma-Aldrich, and the reagents were used
without further purification unless indicated.
(PBS) solutions were prepared using pre-formulated tablets (Sigma-Aldrich)
in 200 mL of Milli-Q water (>18.2 Ω mean resistivity) to
give [NaCl] = 0.138 M, [KCl] = 0.0027 M, and pH 7.4. Poly(methyl vinyl
ether-alt-maleic anhydride) Mn 80 000 and 311 000 g.mol–1, dimethylaminoethanol, and N-[4-(aminomethyl)benzyl]rhodamine
6G-amide bis(trifluoroacetate) were purchased from Sigma-Aldrich.
Poly(methyl vinyl ether-alt-maleic anhydride) average Mn 20 000 g mol–1 was
purchased from Scientific Polymer Products; the dispersity of polymer
were purchased from VWR or Sigma-Aldrich, and the reagents were used
without further purification unless indicated.
4
Polymer-Based Cryopreservation Protocol
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DMSO (analytical reagent grade),
poly(methyl vinyl ether-alt-maleic anhydride) (average
Mn ≈ 80,000 Da, impurities: <2% benzene), Dulbecco’s
phosphate-buffered saline (1.15 g L–1 dibasic sodium
phosphate, 0.2 g L–1 potassium chloride, 8 g L–1 sodium chloride, and 0.2 g L–1 monobasic
potassium phosphate) (DPBS) were purchased from Sigma-Aldrich. Trehalose
dihydrate (purity >98%) was purchased from Carbosynth. Poly(ethylene
glycol) (PEG) (average Mn = 4000 g mol–1) was purchased
from Sigma-Aldrich. 2-Dimethylaminoethanol (purity 99%) was purchased
from Acros Organics. Tetrahydrofuran (laboratory reagent grade) was
purchased from Merck. BD FACSFlow sheath fluid was purchased from
BD biosciences. Sheep’s blood in Alsever’s solution
(not defibrinated) was from TCS Biosciences. Polyampholyte (poly(vinyl
ether-alt-maleic acid mono(dimethylamino ethyl)ester))
was synthesized in house as described in theSupporting Information Milli-Q ultrapure water (>18.2 MΩ cm–1 2 ppb) was used throughout.
poly(methyl vinyl ether-alt-maleic anhydride) (average
Mn ≈ 80,000 Da, impurities: <2% benzene), Dulbecco’s
phosphate-buffered saline (1.15 g L–1 dibasic sodium
phosphate, 0.2 g L–1 potassium chloride, 8 g L–1 sodium chloride, and 0.2 g L–1 monobasic
potassium phosphate) (DPBS) were purchased from Sigma-Aldrich. Trehalose
dihydrate (purity >98%) was purchased from Carbosynth. Poly(ethylene
glycol) (PEG) (average Mn = 4000 g mol–1) was purchased
from Sigma-Aldrich. 2-Dimethylaminoethanol (purity 99%) was purchased
from Acros Organics. Tetrahydrofuran (laboratory reagent grade) was
purchased from Merck. BD FACSFlow sheath fluid was purchased from
BD biosciences. Sheep’s blood in Alsever’s solution
(not defibrinated) was from TCS Biosciences. Polyampholyte (poly(vinyl
ether-alt-maleic acid mono(dimethylamino ethyl)ester))
was synthesized in house as described in the
5
Cytotoxicity Evaluation of Polymeric Compounds
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Advanced Dulbecco’s
Modified Eagle’s
Medium, Ham’s F-12K media, and penicillin/streptomycin/amphotericin
B were obtained from Gibco. Poly(ethylene glycol) (Mn 8 kDa) was obtained from MP Biomedicals. Dimethylaminoethanol,
dimethyl sulfoxide, fetal bovine serum, Dulbecco’s phosphate-buffered
saline, poly(ethylene glycol)methyl ether (Mn 20 kDa), and poly(methyl vinyl ether-alt-maleic anhydride) (Mn 80 kDa) were obtained
from Sigma-Aldrich. A live/dead viability/cytotoxicity kit and CellEvent
Caspase-3/7 Green Detection Reagent were obtained from Thermo Fisher.
All solvents were purchased from VWR or Sigma-Aldrich, and reagents
were used without further purification unless indicated.
Modified Eagle’s
Medium, Ham’s F-12K media, and penicillin/streptomycin/amphotericin
B were obtained from Gibco. Poly(ethylene glycol) (Mn 8 kDa) was obtained from MP Biomedicals. Dimethylaminoethanol,
dimethyl sulfoxide, fetal bovine serum, Dulbecco’s phosphate-buffered
saline, poly(ethylene glycol)methyl ether (Mn 20 kDa), and poly(methyl vinyl ether-alt-maleic anhydride) (Mn 80 kDa) were obtained
from Sigma-Aldrich. A live/dead viability/cytotoxicity kit and CellEvent
Caspase-3/7 Green Detection Reagent were obtained from Thermo Fisher.
All solvents were purchased from VWR or Sigma-Aldrich, and reagents
were used without further purification unless indicated.
6
Biomaterial-Based Growth Factor Delivery
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Soy lecithin, heparin sodium porcine mucosa, ethylenediamine tetrakis(propoxylateblock-ethoxylate) tetrol (Tetronic 1107 ® , T1107), polyoxyethylene (20) (link) sorbitan monolaureate (Tween ® 20), cottonseed oil, polycaprolactone (PCL, Mn 70000 -90000 Da), poly(methyl vinyl ether-alt-maleic) anhydride (PVM/MA, Mn 80000 Da), methoxypolyethylene glycol amine 5000 (PEGAm), dextran sulfate sodium salt (Mw > 500000) were all purchased from Sigma-Aldrich (Missouri, USA). Poly(lactic-coglycolic acid) (PLGA) was obtained from Boehringer-Ingelheim (Petersburg, USA).
Recombinant proteins BMP-7 and TGF-β3 were bought from Peprotech (USA); their respective ELISA kits were bought from Abnova (Taiwan) and Uscn Life Sience Inc.
(China). The remaining reagents and solvents used were of analytical grade or better. In all the experiments, ultrapure water was employed (Milli-Q, Millipore).
Recombinant proteins BMP-7 and TGF-β3 were bought from Peprotech (USA); their respective ELISA kits were bought from Abnova (Taiwan) and Uscn Life Sience Inc.
(China). The remaining reagents and solvents used were of analytical grade or better. In all the experiments, ultrapure water was employed (Milli-Q, Millipore).
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