A Dionex 3000 Ultimate liquid chromatography system coupled to a UV diode array detector (Thermo Fisher, St. Albans, UK) was used using a Phenomenex Synergi Fusion column (150 mm × 2 mm, 4 μm) protected by a 4 mm × 3 mm Phenomenex Synergi Fusion guard column (Phenomenex, Macclesfield, UK). The mobile phases were 70% acetonitrile with 25 mM of triethylammonium phosphate buffer (TEAP) (B) and aqueous TEAP (25 mM) buffer (A). The gradient elution commenced with 4% B and ramped to 70% B in 15 min and held for 3 min, resulting in a total acquisition time of 18 min at a flow rate of 0.6 mL/min. The diode array detection window was set at 200–595 nm (collection rate 2 Hz).
Synergi fusion guard column
Manufactured by Phenomenex
Sourced in United Kingdom
The Phenomenex Synergi Fusion guard column is a high-performance liquid chromatography (HPLC) column designed to protect the analytical column from contaminants and particle buildup. It features a durable, spherical particle design and a proprietary bonded phase that provides reliable performance and extended column lifetime.
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4 protocols using synergi fusion guard column
1
HPLC Analysis of Organic Compounds
2
HPLC-DAD Analysis of Organic Compounds
3
HPLC-DAD Analysis of Compound Mixtures
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HPLC-DAD analyses [19, 20] were carried out on a Dionex 3000 Ultimate system coupled to a UV diode array detector (Thermo Fisher, St Albans, UK), using a Phenomenex Synergi Fusion column (150 mm x 2 mm, 4 µm) that was protected by a 4 mm x 3 mm Phenomenex Synergi Fusion guard column (Phenomenex, Macclesfield, UK). The mobile phases were made from 70% acetonitrile with 25 mM TEAP buffer and an aqueous solution of 25 mM TEAP buffer. Elution was achieved with a gradient that started with 4% acetonitrile and ramped to 70% acetonitrile in 15 min and held for 3 min. The total acquisition time was 18 min at a flow rate of 0.6 mL/min. The diode array detection window was set at 200 nm to 595 nm (collection rate 2 Hz).
4
HPLC-DAD Profiling of Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HPLC-DAD analyses were carried out on a Dionex 3000 Ultimate system coupled to a UV diode array detector (Thermo Fisher, St Albans, UK), using a Phenomenex Synergi Fusion column (150 mm x 2 mm, 4 µm) that was protected by a 4 mm x 3 mm Phenomenex Synergi Fusion guard column (Phenomenex, Macclesfield, UK). The mobile phases were made from 70% acetonitrile with 25 mM triethylammonium phosphate (TEAP) buffer and an aqueous solution of 25 mM TEAP buffer. Elution was achieved with a gradient that started with 4% acetonitrile and ramped to 70% acetonitrile in 15 min and held for 3 min. The total acquisition time was 18 min at a flow rate of 0.6 mL/min. The diode array detection window was set at 200 nm to 595 nm (collection rate 2 Hz).
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