Tissue sections were heated at 95°C for 30 min for antigen retrieval, washed with phosphate-buffered saline (PBS) for 5 min, and blocked in 10% goat serum for 30 min at 37°C. Then, the samples were incubated with rabbit anti-Piezo1 (Abcam, Cambridge, MA, United States, 1:200) overnight at 4°C. On the following day, slides were incubated with the Alexa Fluor 488 goat anti-rabbit (ZF-0511, 1:200, Zhongshan Bio-Tech, Beijing, China) for 1 h at 37°C and counterstained with 4’,6-diamidino-2-phenylindole (DAPI) (Sigma, St. Louis, MO, United States) for 15 min. After washing with PBS, stained sections were observed by using fluorescent microscopy (DMI 6000; Leica, Wetzlar, Germany). Quantitative analyses of the images were done by the Image-Pro Plus 6.0 Software (Media Cybernetics, Bethesda, MD, United States).
Rabbit anti piezo1
Manufactured by Abcam
Sourced in United States
Rabbit anti-Piezo1 is a primary antibody that specifically binds to the Piezo1 protein, a mechanosensitive ion channel. This antibody can be used to detect and study the expression and localization of Piezo1 in various biological samples.
Automatically generated - may contain errors
2 protocols using rabbit anti piezo1
1
Immunofluorescence Analysis of Piezo1 Expression
2
Immunohistochemical Localization of Piezo1 and Piezo2
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Intestinal segments were removed and placed in 4% paraformaldehyde, embedded in paraffin and cut. Immunohistochemistry was performed by standard techniques. The specimens were blocked with 5% bovine serum albumin and then incubated with rabbit anti-Piezo1 (1:250; Abcam, Cambridge, MA, USA) or rabbit anti-Piezo2 (1:250; Abcam) as the primary antibody overnight at 4°C. After incubation with the appropriate biotinylated secondary antibody for 30 minutes at room temperature, the results were visualized using diaminobenzidine (DAB). The densitometry analysis of immunohistochemical staining was performed using the Image-Pro Plus 6.0 software (Version X; Media Cybernetics, Silver Springs, MD, USA).
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