1 × 106 cell suspension from lung digests and BAL were incubated with antibody cocktail containing APC-anti-CD206 (BioLegend. San Diego, CA), PerC-Cy5-anti-F4/80, PE-Cy7-anti-Ly6G, PE-Cy3-anti-p-STAT6, APC-Cy7-anti-CD11b, BV421-anti-Siglec-F (BD Biosciences, Franklin Lakes, NJ and eBioscience, San Diego, CA). After incubation at dark for 40 min, the stained cells were washed for 2 times and analyzed on FACScan cytometer and Flow Jo software, version 8.8.4 (Becton, Dickinson and Company, Franklin Lakes, NJ).
Pe cy3 anti p stat6
Manufactured by BD
PE-Cy3-anti-p-STAT6 is a fluorescently labeled antibody that binds to the phosphorylated form of STAT6, a transcription factor involved in cellular signaling pathways. The PE-Cy3 dye attached to the antibody emits light in the red-orange spectrum when excited, allowing for detection and quantification of phosphorylated STAT6 in various cell-based assays.
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Lab products found in correlation
2 protocols using pe cy3 anti p stat6
1
Immunophenotyping of Lung Immune Cells
2
Inflammatory Cytokine and Cell Profile
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The concentration of TNF-α, IL-6, IL-1β and Calreticulin in BAL or cell supernatants were measured by ELISA assay according to industrial instructions (R&D systems, Minneapolis, MN).
Flow Cytometry analysis 1 × 10 6 cell suspension from lung digests and BAL were incubated with antibody cocktail containing APCanti-CD206 (BioLegend. San Diego, CA), PerC-Cy5-anti-F4/80, PE-Cy7-anti-Ly6G, PE-Cy3-anti-p-STAT6, APC-Cy7-anti-CD11b, BV421-anti-Siglec F (BD Biosciences, Franklin Lakes, NJ and eBioscience, San Diego, CA). The cells stained with uorescence-minus-one (FMO) antibody cocktail were unstained controls. The cells were incubated with antibod cocktail in PBS supplied with 3% FBS for 40 min and washed with PBS for 2 times at room temperature. Analysis was performed on FACScan cytometer and all data were analyzed using FlowJo software, version 8.8.4 (Becton, Dickinson and Company, Franklin Lakes, NJ).
Flow Cytometry analysis 1 × 10 6 cell suspension from lung digests and BAL were incubated with antibody cocktail containing APCanti-CD206 (BioLegend. San Diego, CA), PerC-Cy5-anti-F4/80, PE-Cy7-anti-Ly6G, PE-Cy3-anti-p-STAT6, APC-Cy7-anti-CD11b, BV421-anti-Siglec F (BD Biosciences, Franklin Lakes, NJ and eBioscience, San Diego, CA). The cells stained with uorescence-minus-one (FMO) antibody cocktail were unstained controls. The cells were incubated with antibod cocktail in PBS supplied with 3% FBS for 40 min and washed with PBS for 2 times at room temperature. Analysis was performed on FACScan cytometer and all data were analyzed using FlowJo software, version 8.8.4 (Becton, Dickinson and Company, Franklin Lakes, NJ).
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