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Alexa fluor 568 conjugated mouse secondary antibody

Manufactured by Thermo Fisher Scientific
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Alexa Fluor® 568 conjugated mouse secondary antibody is a fluorescently labeled antibody that can be used to detect and visualize mouse primary antibodies in various experimental techniques, such as immunofluorescence, flow cytometry, and Western blotting. The Alexa Fluor® 568 dye provides a bright, photostable signal that can be detected using standard fluorescence microscopy or flow cytometry equipment.

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Lab products found in correlation

4 6 diamidino 2 phenylindole dihydrochloride dapi, by Thermo Fisher Scientific (2 mentions) Dmirb epi fluorescent microscope, by Leica (2 mentions) Sma primary antibody, by Merck Group (2 mentions) Sp8 confocal microscope, by Leica (2 mentions)

Close spelling variants of this product

Alexa Fluor-conjugated secondary antibodies (1427 citations) Alexa Fluor 568 (1190 citations) Alexa Fluor secondary antibodies (639 citations) Alexa 568 (337 citations) Alexa-conjugated secondary antibodies (274 citations) Alexa Fluor 568-conjugated secondary antibody (118 citations) Alexa Fluor 568 secondary antibody (65 citations) Alexa 568-conjugated secondary antibody (25 citations) Alexa Fluors (21 citations) Alexa Fluor 568-conjugated goat anti-mouse secondary antibody (15 citations)

2 protocols using alexa fluor 568 conjugated mouse secondary antibody

1

Immunofluorescence Analysis of Smooth Muscle Actin

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Smooth muscle actin (SMA) expression in explanted cells was analyzed by immunofluorescence. SMA primary antibody (Sigma A2547, dilution 1:500) was diluted in 2% bovine serum albumin (BSA; EMD Chemicals, San Diego, CA) in PBS. Primary antibodies were detected using 1:500 Alexa Fluor® 568 conjugated mouse secondary antibody (Life Technologies A11004, Waltham, MA) and nuclei were detected with 1:10,000 4’,6-diamidino-2-phenylindole,dihydrochloride (DAPI; Life Technologies D1306) diluted in 2% BSA in PBS. Imaging was performed on a Leica SP8 confocal microscope (Wetzlar, Germany) or a Leica DMIRB epi-fluorescent microscope (Wetzlar, Germany).
Davis-Knowlton J., Turner J.E., Turner A., Damian-Loring S., Hagler N., Henderson T., Emery I.F., Bond K., Duarte C.W., Vary C.P., Eldrup-Jorgensen J, & Liaw L. (2018). Characterization of smooth muscle cells from human atherosclerotic lesions and their responses to Notch signaling. Laboratory investigation; a journal of technical methods and pathology, 99(3), 290-304.
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2

Immunofluorescence Analysis of Smooth Muscle Actin

Check if the same lab product or an alternative is used in the 5 most similar protocols
Smooth muscle actin (SMA) expression in explanted cells was analyzed by immunofluorescence. SMA primary antibody (Sigma A2547, dilution 1:500) was diluted in 2% bovine serum albumin (BSA; EMD Chemicals, San Diego, CA) in PBS. Primary antibodies were detected using 1:500 Alexa Fluor® 568 conjugated mouse secondary antibody (Life Technologies A11004, Waltham, MA) and nuclei were detected with 1:10,000 4’,6-diamidino-2-phenylindole,dihydrochloride (DAPI; Life Technologies D1306) diluted in 2% BSA in PBS. Imaging was performed on a Leica SP8 confocal microscope (Wetzlar, Germany) or a Leica DMIRB epi-fluorescent microscope (Wetzlar, Germany).
Davis-Knowlton J., Turner J.E., Turner A., Damian-Loring S., Hagler N., Henderson T., Emery I.F., Bond K., Duarte C.W., Vary C.P., Eldrup-Jorgensen J, & Liaw L. (2018). Characterization of smooth muscle cells from human atherosclerotic lesions and their responses to Notch signaling. Laboratory investigation; a journal of technical methods and pathology, 99(3), 290-304.
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