Colonic or spleen tissue sections (4 μm) were routinely dewaxed and hydrated with gradient ethanol. The samples were treated with antigen repair solution at 95–99°C for 40 min and cooled at room temperature for 20 min. After washing 3 times, CD86 antibody (ABclonal, Wuhan, China; A2353; 1 : 50) or CD206 (Servicebio, Wuhan, China; GB113497; 1 : 100) was added and incubated overnight at 4°C. Then, the EnVision detection and color development kit was used for DAB color development, hematoxylin restaining, gradient ethanol dehydration, xylene transparency, and treacle sealing for observation. IHC images were evaluated microscopically (BA400Digital, Motic Instruments, Inc., Baltimore, MD, USA).
Cd86 antibody
Manufactured by ABclonal
Sourced in China
The CD86 antibody is a laboratory reagent used for the detection and analysis of the CD86 protein, which is a costimulatory molecule expressed on the surface of antigen-presenting cells. This antibody can be used in various immunological techniques to study the expression and function of CD86 in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Cd206, by Wuhan Servicebio Technology (1 mentions)
Bovine serum albumin (bsa), by Biosharp (1 mentions)
Horseradish peroxidase conjugated goat anti rabbit igg, by Proteintech (1 mentions)
Nf κb p p65 antibody, by Affinity Biosciences (1 mentions)
Mcp 1 antibody, by ABclonal (1 mentions)
Ripa buffer, by Beyotime (1 mentions)
Bca protein assay kit, by Beyotime (1 mentions)
2 protocols using cd86 antibody
1
Immunohistochemistry Analysis of CD86 and CD206
2
Western Blot Analysis of Immune Regulators
Check if the same lab product or an alternative is used in the 5 most similar protocols
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As for Western blot, the whole proteins were extracted with lysate buffer (RIPA buffer, Beyotime). Quantification was accomplished by the BCA protein assay kit (Beyotime). After that, the proteins were electrophoresed in a 10% SDS-PAGE gels electrophoresis for separation and transferred onto the polyvinylidene fluoride membrane. Blocking buffer was prepared with 5% bovine serum albumin (Biosharp, Anhui, China). Then, they were immunoblotted with the primary antibodies (1:1,000) and detected with horseradish peroxidase-conjugated goat anti-rabbit-IgG (1:10,000, Proteintech, Wuhan, China). Then, the membranes were visualized by using Gel-Pro-Analyzer Software (Beijing, China). The primary antibodies involved in this procedure are listed as follows: LAPTM5 antibody, MCP1 antibody, and CD86 antibody were obtained from ABclonal (China). nuclear factor-kappa B (NF-κB) p65 antibody, NF-κB p-p65 antibody, p-IκBα antibody, and IκBα antibody were purchased from Affinity (Jiangsu, China).
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