Acridine orange ethidium bromide

Sodium alginate (medium viscosity, Sigma, Saint Louis, MO, USA), chitosan (low molecular weight, ≥75% deacetylation, Sigma), Thiazolyl Blue Tetrazolium Bromide (Sigma), and sodium triphosphate (TPP, Merck, Rahway, NJ, USA) were purchased from a local supplier (Alborz Shimi Co., Tehran, Iran). Phosphate-buffered saline, 4,6-diamidino-2-phenylindole dihydrochloride (Invitrogen, Waltham, MA, USA), acridine orange ethidium bromide (Invitrogen) were provided by KalaZist Co. (Tehran, Iran). All reagents were analytical grades.

The suppliers of the following compounds are as follows: Winfield red cigarettes (Phillip Morris, Australia); ebselen (Sapphire Bioscience, Australia); CM‐cellulose (Sigma‐Aldrich, USA); sodium pentabarbitone (Virbac, Australia); acridine orange/ethidium bromide (Invitrogen, USA); Kwik‐Diff® reagent 1, fixative (Thermo Fisher Scientific, USA); Hemacolour® rapid staining of blood smear (eosin solution) (Merck, USA); Hemacolour® rapid staining of blood smear (thiazine solution) (Merck, USA); RNeasy Mini Kit (Qiagen, Germany); High Capacity RNA‐to‐cDNA kit (Thermo Fisher Scientific, USA); pre‐developed TaqMan primers to: TNF‐α (ID: Mm00443258_m), IL‐6 (ID: Mm00446190_m1), NOX‐2/CYBB (ID: Mm01287743_m1), GPx‐1 (ID: Mm00656767_g1) (Thermo Fisher Scientific, USA); U46619 (Cayman Chemical, USA); ACh (Thermo Fisher, USA); sodium nitroprusside (Thermo Fisher, USA); eNOS/NOS3 RRID: AB_2533121 (Thermo Fisher Scientific, USA), 3‐NT RRID: AB110282 (Abcam, USA); Goat anti‐mouse IgG (H + L) secondary antibody, Alexa Flour Plus 488 RRID: AB_2633275 (Thermo Fisher Scientific, USA); Fluoromount‐G™, with DAPI (Thermo Fisher Scientific, USA).

The suppliers of the materials used in these experiments are as follows: Winfield Red cigarettes (Phillip Morris, Australia); apocynin and DMSO (Sigma‐Aldrich, Australia); ketamine/xylazine and sodium pentobarbitone (Virbac Pty Ltd, Australia); acridine orange/ethidium bromide (Invitrogen, USA); Kwik‐Diff® reagent 1 fixative, High‐Capacity RNA‐to‐cDNA kit, pre‐developed TaqMan primers, cell culture reagents, Fluoromount‐G™, with DAPI, murine IL‐6 ELISA Kit, Pierce™ BCA Protein Assay Kit and SuperSignal™ West Femto Maximum Sensitivity Substrate for chemiluminescence detection (Thermo Fisher Scientific, USA);
RNeasy Mini Kit (Qiagen, Germany); C2C12 murine myoblasts (American Type Culture Collection, USA; CRL‐1772); H₂O₂ (Chem‐Supply, Australia); antibody for immunofluorescence (Santa Cruz Biotechnology, USA); MTS Cell Proliferation Assay (Promega, Australia); murine IGF‐1 DuoSet ELISA Kit (R&D Systems, USA); phosphorylation‐specific, actin antibodies and p62 for western blots (Cell Signaling Technology, USA); all other antibodies for western blots (Abcam, USA).